SEMA4D ELISA Kit (Mouse) (OKCD02880)

Catalog No: OKCD02880

Size:96WELLS

Mouse SEMA4D ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	78-90%	87-94%	99-105%
  EDTA Plasma (n=5)	94-105%	83-98%	92-101%	88-96%
  Heparin Plasma (n=5)	80-91%	94-103%	82-90%	89-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 4D (SEMA4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 4D (SEMA4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 4D (SEMA4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Semaphorin 4D (SEMA4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-98	88
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma(n=5)	88-102	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 4D (SEMA4D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SEMA4D Microplate: 96 Wells (12 x 8 Well strips)
  • SEMA4D Lyophilized Standard: 2
  • 100X Biotinylated SEMA4D Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.30 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D). No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Sema4d
• Gene Full Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Alias Symbols: CD100;col;coll-4;M-sema G;Sem;Sema;sema J;Semacl2;Semaj;semaphorin H;semaphorin-4D;semaphorin-C-like 2;semaphorin-J;Semc;Semcl2.
• NCBI Gene Id: 20354
• Protein Name: Semaphorin-4D
• Description of Target: Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA (By similarity).
• Uniprot ID: O09126
• Protein Accession #: NP_001268809.1
• Nucleotide Accession #: NM_001281880.1
• Protein Size (# AA): 861