Size:96 Wells
$589.00
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SKU
OKCD02880
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SEMA4D ELISA Kit (Mouse) (OKCD02880)

Catalog#: OKCD02880
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species ReactivityMouse
Kit Detection MethodColorimetric, OD450 nm
Kit Duration3 Hours
Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%78-90%87-94%99-105%
EDTA Plasma (n=5)94-105%83-98%92-101%88-96%
Heparin Plasma (n=5)80-91%94-103%82-90%89-102%
Kit PrincipleAviva Systems Biology Sema4d ELISA Kit (Mouse) (OKCD02880) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Sema4d has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Sema4d is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sema4d captured in well.
Kit Range0.78-50 ng/mL
Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-9888
EDTA Plasma (n=5)98-105102
Heparin Plasma(n=5)88-10291
Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level SEMA4D was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level SEMA4D was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Component
ComponentAmount
Anti-SEMA4D Microplate96 Wells (12 x 8 Well strips)
SEMA4D Lyophilized Standard2
100X Biotinylated SEMA4D Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.30 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityThis assay has high sensitivity and excellent specificity for detection of Semaphorin 4D (SEMA4D). No significant cross-reactivity or interference between Semaphorin 4D (SEMA4D) and analogues was observed
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSEMA4D
Official Gene Full Namesema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Alias SymbolsCD100, coll-4, M-sema G, M-Sema G, Semacl2, Semaj, Sema J, Semaphorin-4D, Semaphorin-C-like 2, semaphorin H, Semaphorin-J, Semcl2
NCBI Gene Id20354
Protein NameSemaphorin-4D
Description of TargetCell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
Swissprot IdO09126
Protein Accession #NP_001268809.1
Nucleotide Accession #NM_001281880.1
Protein Size (# AA)861
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express SEMA4D.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express SEMA4D.
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