- NCBI Gene Id:
- Alias Symbols:
- Semaphorin-4D, A8, BB18, GR3, CD100, SEMA4D, C9orf164, CD100, SEMAJ
- Replacement Item:
- This antibody may replace item sc-136250 from Santa Cruz Biotechnology.
- Description of Target:
- Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
- Molecular Weight:
- 96 kDa
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express SEMA4D.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express SEMA4D.
- This SEMA4D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 793-832 amino acids from the C-terminal region of human SEMA4D.
- Predicted Species Reactivity:
- Product Format:
- Liquid. PBS with 0.09% (W/V) sodium azide.
- Reconstitution and Storage:
- Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
- 0.5 mg/ml
- Rabbit Ig
- Application Info:
- WB: 1:2000
- Cellular Location: Cell membrane; Single-pass type I membrane protein.
Tissue Location: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
- Target Reference:
- Hall K.T.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:11780-11785(1996).
Humphray S.J.,et al.Nature 429:369-374(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Tamagnone L.,et al.Cell 99:71-80(1999).
Basile J.R.,et al.Mol. Cell. Biol. 25:6889-6898(2005).