Size:96 Wells
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SEMA4D ELISA Kit (Human) : 96 Wells (OKEH01612)

Catalog#: OKEH01612
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology SEMA4D ELISA Kit (Human) (OKEH01612) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEMA4D has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEMA4D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SEMA4D captured in well.
Kit Range0.78 - 50ng/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Kit ReproducibilityMean Intra-assay CV%: < 6.8% (n = 20)
Mean Inter-assay CV%: < 10.4% (n = 20)
Kit Component
SEMA4D Microplate96 Wells (12 x 8 Well strips)
SEMA4D Lyophilized Standard2
100X Biotinylated SEMA4D Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.63 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Human Semaphorin-4D
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolSEMA4D
Official Gene Full Namesemaphorin 4D
Alias SymbolsA8, BB18, C9orf164, CD100, coll-4, FLJ33485, FLJ34282, FLJ39737, FLJ46484, GR3, MGC169138, MGC169141, M-sema-G, SEMAJ, Semaphorin-4D
NCBI Gene Id10507
Protein NameSemaphorin-4D
Swissprot IdQ92854
Protein Accession #NP_001135759.1
Nucleotide Accession #NM_001142287.1
Protein Size (# AA)862
Molecular Weight96 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express SEMA4D.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express SEMA4D.
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