SEMA4D Antibody - middle region (OAAB19369)

Catalog No: OAAB19369

Size:400ul

Anti-SEMA4D Antibody (Center)at 1: 2000 dilution + HL-60 whole cell lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 96 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
  Cellular Location: Cell membrane; Single-pass type I membrane protein.
  Tissue Location: Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SEMA4D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 553-587 amino acids from the Central region of human SEMA4D.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Application Info: WB: 1:2000
• Reference: Hall K.T.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:11780-11785(1996).
  Humphray S.J.,et al.Nature 429:369-374(2004).
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Tamagnone L.,et al.Cell 99:71-80(1999).
  Basile J.R.,et al.Mol. Cell. Biol. 25:6889-6898(2005).
  

Target Info

• Gene Symbol: SEMA4D
• Alias Symbols: A8, GR3, BB18, CD100, COLL4, SEMAJ, coll-4, C9orf164, M-sema-G
• NCBI Gene Id: 10507
• Description of Target: Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
• Uniprot ID: Q92854
• Molecular Weight: 96 kDa