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Enahnced Validation Products By Aviva Systems Biology

Aviva's Methods of ENHANCED VALIDATION

Aviva Systems Biology is committed to ensuring the quality and reliability of our primary antibody portfolio

Addressing the Crisis of Reproducibility


Researchers face the pains of inconsistent results when using antibody-based techniques. A major contributing factor to this lack of reproducibility is inadequate standardization and validation by antibody manufacturers.

While it’s important for individual researchers to validate the antibodies they use in their samples, manufacturers should do better validations up front to save researchers valuable time and resources! The bottom-line in improving antibody-based research tools requires standardization of antibody validation, as well as increased transparency and sharing information about antibody performance.

Aviva is among those antibody suppliers that embody these principles; advocating for standardization and validation rigor. Learn more about the methods of Enhanced Validation that we have adopted below, and look for our antibodies that carry the Aviva Badge of Enhanced Validation.

Genetic Validation

Genetic
Validation

  • The most trusted method of validation
  • With proper controls, a genetic knockout, in which complete inactivation or deletion of a gene in an organism is achieved, provides powerful evidence for antibody specificity through a apparent loss of signal
  • A knockdown model, resulting in partial reduction of gene expression, also gives rise to valuable information in which the level of knockdown can be quantified
  • Aviva is a proud partner with the YCharOS (Antibody Characterization through Open Science) initiative see our Knockout-validated TIA1 Antibody - C-terminal region (ARP40981_P050).

SPR Affinity
Characterization

  • Surface plasmon resonance (SPR) is a powerful technique used to analyze protein-protein interactions in real-time.
  • SPR can be used to confirm the affinity of an antibody for its target protein by quantifying rates at which the antibody binds its target, ie the association and dissociation constants ka and kd
  • Provides a quantitative understanding of the antibody’s kinetic properties and interactions with its target protein
  • Learn more about SPR here.

 Biological Expression

Biological
Expression

Independent Recognition

Independent
Recognition

  • Using two different antibodies against the same target and showing that both give positive signal is an important step in antibody validation
  • Multiple epitope/independent recognition is the second-most trusted method of antibody validation
  • Comparing the Western Blotting results obtained with two independent antibodies for the same target can shed light on any non-specific binding or cross-reactivity that may occur with one of the antibodies

Aviva-validated antibodies

Save time and money with the confidence of Aviva's Enhanced Validated antibodies