Western Blot Reagents

Quality western blot results rely both on high specificity and quality antibodies as well as reliable secondaries and detection reagents such as enhanced chemiluminescent substrates. Aviva Systems Biology offers a broad range of products for standard western blot/immunoblot detection formulated to address specific customer needs, such as range of performance, stability and duration of signal, and ease of use. Select the category of western blot products you need below!

NEW! AvivaBlot™ ECL Reagents

High Quality, Affordable Reagents for Western Blotting

The new AvivaBlot products are stabilized formulations that allow room temperature shipping and storage for up to 1 year from date of receipt. Try one of our high-performance simplified solutions today, offered with secondaries, loading controls and epitope tag antibodies to support general western blot detection needs from loading controls to rare, low femtogram containing protein samples.

Select from One of Three Sensitivity Ranges

Description Cat. No. Details
AvivaBlot Pico One ECL Reagent OKCF00001 One component, pre-mixed. Great for general detection needs.
AvivaBlot Femto ECL Reagent OKCF00002 Two component. High range flexibility, mid-femtogram detection.
AvivaBlot Ultra Femto ECL Reagent OKCF00003 Two component. Low femtogram detection for rare and precious samples.

Benefits of AvivaBlot ECL Reagents

  • Competitive performance ensures AvivaBlot reagents will perform as well or better than your current western blot reagents.
  • Simple portfolio for easy selection of reagents that will support the detection of the proteins you study.
  • Room temperature storage eliminates the need for any special storage or shipping conditions.
  • Stable, premixed single solution available with AvivaBlot Pico One ECL Reagent.
  • Affordable pricing provides the most reagent per dollar.

Extended Duration of AvivaBlot Femto ECL Reagent (OKCF00002)

Quadruplicate blots for each substrate containing 2-fold dilutions of HeLa whole cell lysate were incubated with Rabbit-anti Human HDAC-1 1:5000 Goat anti Rabbit-HRP 1: 75000 and were simultaneously imaged with ImageQuant™ LAS 4000 (GE Healthcare) at time points up to 20 hours post substrate addition.

Quadruplicate blots for each substrate containing 2-fold dilutions of HeLa whole cell lysate were incubated with Rabbit-anti Human HDAC-1 1:5000 Goat anti Rabbit-HRP 1: 75000 and were simultaneously imaged with ImageQuant™ LAS 4000 (GE Healthcare) at time points up to 20 hours post substrate addition.

 


AvivaBlot Pico One ECL Reagent (OKCF00001)

Western blot ECL comparison using Anti-HDAC1 at 1:2,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.078 ug).)

Western blot ECL comparison using Anti-HDAC1 at 1:2,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.078 ug).

 

 

  • One component, premixed, and ready to use
  • Room temperature storage
  • Mid-picogram detection
  • Ideal for everyday detection needs
  • 250 mL size for 35 mini or 22 midi gels


AvivaBlot Femto ECL Reagent (OKCF00002)

“Western blot ECL comparison using Anti-HDAC1 at 1:5,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.156 ug).”

Western blot ECL comparison using Anti-HDAC1 at 1:5,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.156 ug).

 

 

  • Two components
  • Working solution is stable for 3 days
  • Room temperature storage
  • High range flexibility, mid-femtogram detection
  • Extended signal duration
  • 250 mL size for 35 mini or 22 midi gels


AvivaBlot Ultra Femto ECL Reagent (OKCF00003)

Western blot ECL comparison using Anti-HDAC1 at 1:10,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 2.5 ug to 0.039 ug).

Western blot ECL comparison using Anti-HDAC1 at 1:10,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 2.5 ug to 0.039 ug).

 

 

  • Two components
  • Working solution is stable for 3 days
  • Low femtogram detection
  • Market leading sensitivity for rare and precious samples
  • 100 mL size for 15 mini or 9 midi gels