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Western Blot Reagents

To obtain exceptional results in your Western blot experiments, various factors come into play. The antibodies you choose are particularly crucial, as their specificity and quality are paramount. Additionally, the use of dependable secondary antibodies and sensitive detection reagents, such as enhanced chemiluminescent substrates, is equally important.

We understand the significance of these components in your data collection. That's why we are dedicated to offering a comprehensive range of products specifically tailored for Western blot and immunoblot detection. We recognize that each researcher has unique requirements, which is why our product collection encompasses a variety of options.

Whether you need antibodies with broad species reactivity or ECL products with enhanced stability and longer signal duration, Aviva Systems Biology has you covered! We are committed to equipping researchers like you with the necessary tools to achieve consistent and reliable results in your Western blot experiments. Explore our wide selection of Western blot products below and choose the ones that best align with your research objectives. We are here to support your scientific journey and assist you in reaching new heights in your research. If you have any questions or need further assistance, please don't hesitate to reach out to our knowledgeable team. Your success is our top priority!

NEW! AvivaBlot™ ECL Reagents

High Quality, Affordable Reagents for Western Blotting

The new AvivaBlot products are stabilized formulations that allow room temperature shipping and storage for up to 1 year from date of receipt. Explore our range of high-performance simplified solutions today!

Benefits of AvivaBlot ECL Reagents

  • Competitive performance ensures AvivaBlot reagents will perform as well or better than your current western blot reagents.
  • Simple portfolio for easy selection of reagents that will support the detection of the proteins you study.
  • Room temperature storage eliminates the need for any special storage or shipping conditions.
  • Stable, premixed single solution available with AvivaBlot Pico One ECL Reagent.
  • Affordable pricing provides the most reagent per dollar.
Description Cat. No. Details
AvivaBlot Pico One ECL Reagent OKCF00001 One component, pre-mixed. Great for general detection needs.
AvivaBlot Femto ECL Reagent OKCF00002 Two component. High range flexibility, mid-femtogram detection.
AvivaBlot Ultra Femto ECL Reagent OKCF00003 Two component. Low femtogram detection for rare and precious samples.

Benefits of AvivaBlot ECL Reagents

  • Competitive performance ensures AvivaBlot reagents will perform as well or better than your current western blot reagents.
  • Simple portfolio for easy selection of reagents that will support the detection of the proteins you study.
  • Room temperature storage eliminates the need for any special storage or shipping conditions.
  • Stable, premixed single solution available with AvivaBlot Pico One ECL Reagent.
  • Affordable pricing provides the most reagent per dollar.

 

Extended Duration of AvivaBlot Femto ECL Reagent (OKCF00002)

Quadruplicate blots for each substrate containing 2-fold dilutions of HeLa whole cell lysate were incubated with Rabbit-anti Human HDAC-1 1:5000 Goat anti Rabbit-HRP 1: 75000 and were simultaneously imaged with ImageQuant™ LAS 4000 (GE Healthcare) at time points up to 20 hours post substrate addition.

 

Quadruplicate blots for each substrate containing 2-fold dilutions of HeLa whole cell lysate were incubated with Rabbit-anti Human HDAC-1 1:5000 Goat anti Rabbit-HRP 1: 75000 and were simultaneously imaged with ImageQuant™ LAS 4000 (GE Healthcare) at time points up to 20 hours post substrate addition.

 


AvivaBlot Pico One ECL Reagent (OKCF00001)

Western blot ECL comparison using Anti-HDAC1 at 1:2,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.078 ug).)

Western blot ECL comparison using Anti-HDAC1 at 1:2,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.078 ug).

 

 

  • One component, premixed, and ready to use
  • Room temperature storage
  • Mid-picogram detection
  • Ideal for everyday detection needs
  • 250 mL size for 35 mini or 22 midi gels

 


AvivaBlot Femto ECL Reagent (OKCF00002)

“Western blot ECL comparison using Anti-HDAC1 at 1:5,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.156 ug).”

Western blot ECL comparison using Anti-HDAC1 at 1:5,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 5 ug to 0.156 ug).

 

 

 

  • Two components
  • Working solution is stable for 3 days
  • Room temperature storage
  • High range flexibility, mid-femtogram detection
  • Extended signal duration
  • 250 mL size for 35 mini or 22 midi gels

 


AvivaBlot Ultra Femto ECL Reagent (OKCF00003)

Western blot ECL comparison using Anti-HDAC1 at 1:10,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 2.5 ug to 0.039 ug).

Western blot ECL comparison using Anti-HDAC1 at 1:10,000 on duplicate blots of HeLa whole cell lysates (2-fold dilutions from 2.5 ug to 0.039 ug).

 

 

  • Two components
  • Working solution is stable for 3 days
  • Low femtogram detection
  • Market leading sensitivity for rare and precious samples
  • 100 mL size for 15 mini or 9 midi gels