Rat anti-Human CD3 Antibody (OASA11552)

Catalog No: OASA11552 (Formerly GWB-Q01401)

Size:25UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: CD3-12
• Isotype: IgG1
• Host: Rat
• Application: Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin
• Additional Information: Histology Positive Control Tissue: Tonsil
  Carrier Free: Yes
• Reconstitution and Storage: -20°C
• Immunogen: Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
• Purification: Purified IgG prepared from tissue culture supernatant
• Predicted Homology Based on Immunogen Sequence: Bovine, dog, horse, Rhesus monkey, pig, chicken, mouse, duck, koala, Harbour porpoise, alpaca, Cynomolgus monkey, spotted hyena, sea lion, cat, Amazon parrot, raccoon, great horned-owl, bullfrog, xenopus, rabbit. Based on sequence similarity, is expected to react with: mammals, birds, amphibia. Antibody reactivity and working conditions may vary between species. Antibody reactivity and working conditions may vary between species.
• Concentration: 1 mg/ml
• Specificity: Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3-epilson chain recognizes human CD3-epsilon. As this product has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker.
• Application Info: FC: 1/50-1/100. Membrane permeabilization is required for this application
  IHC-F: 1/100
  IHC-P: 1/100. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer (pH 9.0) is recommended for this purpose.
• Publications:

  Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System. J Neuropathol Exp Neurol. 74, 791-803 (2015). 26115192

  Natalizumab-associated progressive multifocal leukoencephalopathy in a patient with multiple sclerosis: a postmortem study. J Neuropathol Exp Neurol. 72, 1043-51 (2013). 24128680

• Reference: 1. Jones, M. et al. (1993). Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J. Immunol. 150 (12): 5429-5435.
  2. Alterio, M. et al. (1998). Control of cytomeglavirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rest primarily on recipient- derived CD8 T cells. J. Virol. 72 (10): 7733 - 7744
  3. Burundi, E. et al. (2002). Regulation of indoeamine 2, 3-diooxygenase expression in Simian Immunodefiency Virus-infected monkey brains. J. Virol. 76 (3): 12233 - 12241.
• Description:
  View product citations for Rat anti-Human CD3 Antibody (OASA11552) on CiteAb

Target Info

• Alias Symbols: T3E, TCRE, IMD18
• NCBI Gene Id: 916
• Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Description of Target: CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta). The CD3 complex serves as a T-cell co-receptor that associates non-covalently with the T-cell receptor (TCR). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as a T-cell marker.
• Uniprot ID: P07766
• Protein Accession #: NP_000724.1