Catalog No: OKCF00001
Size:250 mL
Price: $130.00
SKU
OKCF00001
Availability: Domestic: 1-2 days delivery International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual.
Click here to download short protocol.
Product Info
Product FormatLiquid
ApplicationWB
Additional InformationOne 10 mL or 250 mL bottle of Avivablot Pico One ECL Reagent (OKCF00001) pre-mixed and ready to use.
StorageStore at Room Temperature
Protocol Information
  1. Perform electrophoresis, membrane transfer and antibody incubation and washes.
  2. Apply Pico One room temperature substrate directly to semi-wet blot using 1 mL per 10 cm3 of membrane. Standard sized mini blots are ~64 cm2 requiring ~7 mLs of solution.
  3. Incubate 2 minutes at RT.
  4. Expose membrane using chemiluminescent imager or x-ray film. Use plastic wrap to avoid wetting film where appropriate.
  5. Exposure time is empirically determined, typical exposures require 60 to 180 seconds.
DilutionPrimary: 1:100 - 1:5000 Secondary: 1:1000 - 1:20000 Varies with chosen antibodies, dilutions from 1 mg/mL stock
Intended UseChemiluminescent substrate for Horseradish Peroxidase based Western Blot detection.
Description

AvivaBlot™ Pico One ECL Reagent is an all in one enhanced chemiluminescent detection reagent, formulated pre-mixed and ready to use for immunoblot applications. Available as sample size (10 mL) for a single application (100 cm2) or as a 250 mL bottle for 2500 cm2, sufficient for approximately 35 mini gel applications. The signal duration is 3 hours, and the reagent can be stored at room temperature for up to 1 year from date of receipt. Primary antibodies are generally used at 1:100-1:5000 dilution range, and secondary antibodies from 1:1000 to 1:20,000 from 1 mg/mL stock solutions.

Alias SymbolsOKCF00001, immunoblot, western blot, blot, ECL, HRP, substrate, "Aviva Blot"
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