Catalog No: OKCF00001
Size:250 mL
Price: $130.00
SKU
OKCF00001
Availability: Domestic: In stock - WB reagents International: In stock - WB reagents
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual.
Click here to download short protocol.
Product Info
Product FormatLiquid
ApplicationWB
Additional InformationOne 10 mL or 250 mL bottle of Avivablot Pico One ECL Reagent (OKCF00001) pre-mixed and ready to use.
StorageStore at Room Temperature
Protocol Information
  1. Perform electrophoresis, membrane transfer and antibody incubation and washes.
  2. Apply Pico One room temperature substrate directly to semi-wet blot using 1 mL per 10 cm3 of membrane. Standard sized mini blots are ~64 cm2 requiring ~7 mLs of solution.
  3. Incubate 2 minutes at RT.
  4. Expose membrane using chemiluminescent imager or x-ray film. Use plastic wrap to avoid wetting film where appropriate.
  5. Exposure time is empirically determined, typical exposures require 60 to 180 seconds.
DilutionPrimary: 1:100 - 1:5000 Secondary: 1:1000 - 1:20000 Varies with chosen antibodies, dilutions from 1 mg/mL stock
Intended UseChemiluminescent substrate for Horseradish Peroxidase based Western Blot detection.
Alias SymbolsOKCF00001, immunoblot, western blot, blot, ECL, HRP, substrate, "Aviva Blot"
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