AvivaBlot Ultra Femto ECL Reagent (OKCF00003)

Catalog No: OKCF00003

Size:100 mL

AvivaBlot™ Ultra Femto Competitor Analysis. Hela whole cell lysates were 2 fold serially diluted from 2.5 ug/lane to 0.039 ug/lane Total Protein. An Anti-HDAC1 antibody was used at 1:10,000, with a Goat anti-Rabbit IgG HRP used at 1:300,000. Exposure time of 120 seconds on an GE Imagequant LAS 4000 was used for image acquisition.

AvivaBlot™ Ultra Femto Signal to Noise Comparison. Triplicate blots containing 2 fold dilutions of HeLa cell lysate were incubated with Rabbit anti-Human HDAC1 1:15,000 and Goat anti-Rabbit IgG HRP 1:300,000 followed by imaging for 120 seconds with Imagequant LAS 4000 (GE Healthcare). Signal to noise measurements are shown for Utra Femto versus leading Competitor A.

AvivaBlot™ Ultra Femto Precision. Western blotting detection of HDAC-1 in HeLa cell lysates with Avivablot Femto. Triplicate blots containing 2 fold dilutions of whole cell lysates were incubated with anti-HDAC1 (Human) at 1:15,000, and Goat anti-Rabbit-IgG HRP at 1:300,0000. Blots were imaged for 120 seconds with ImageQuant LAS 40000 (GE Healthcare). Integrated averaged signal intensity, showing linear response with amount of lysate over a wide range (DR = Dynamic Range, R2 = linearity).

AvivaBlot™ Ultra Femto Product Features

Product Info

• Application: WB
• Additional Information:
  1. Avivablot Ultra Femto Part A (5 mL or 50 mL), amber bottle, store at Room Temperature
  2. Avivablot Femto Part B (5 mL or 50 mL), store at Room Temperature
  Mix 1:1 volume/volume prior to use
• Storage: Store at Room Temperature
• Protocol Information:
  1. Perform electrophoresis, membrane transfer and antibody incubation and washes.
  2. Mix Ultra Femto Part A and Ultra Femto Part B 1:1 in a conical tube thoroughly. 7 mLs final is required for a standard mini blot (~64 cm2 ). Working solutions can be stored at 4° C for up to 3 days but should be warmed to room temperature before use.
  3. Apply Ultra Femto working solution at room temperature directly to semi-wet blot using 1 mL per 10 cm3 of membrane.
  4. Incubate 2 minutes at RT.
  5. Expose membrane using chemiluminescent imager or x-ray film. Use plastic wrap to avoid wetting film where appropriate.
  6. Exposure time is empirically determined, typical exposures require 60 to 180 seconds.
• Dilution: Primary: 1:5.000 - 1:100.000 Secondary: 1:100.000 - 1:500.000 Varies with chosen antibodies, dilutions from 1 mg/mL stock
• Intended Use: Chemiluminescent substrate for Horseradish Peroxidase based Western Blot detection.

Target Info

• Alias Symbols: OKCF00003, immunoblot, western blot, blot, ECL, HRP, substrate, "Aviva Blot"