| Product Number |
OASA11552 |
| Product Page |
www.avivasysbio.com/rat-anti-human-cd3-antibody-oasa11552.html |
| Name |
Rat anti-Human CD3 Antibody (OASA11552) |
| Clone |
CD3-12 |
| Isotype |
IgG1 |
| NCBI Gene Id |
916 |
| Host |
Rat |
| Clonality |
Monoclonal |
| Concentration |
1 mg/ml |
| Description |
|
| Alias Symbols |
T3E, TCRE, IMD18 |
| Product Format |
Liquid. Phosphate buffered saline |
| Reference |
1. Jones, M. et al. (1993). Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J. Immunol. 150 (12): 5429-5435.
2. Alterio, M. et al. (1998). Control of cytomeglavirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rest primarily on recipient- derived CD8 T cells. J. Virol. 72 (10): 7733 - 7744
3. Burundi, E. et al. (2002). Regulation of indoeamine 2, 3-diooxygenase expression in Simian Immunodefiency Virus-infected monkey brains. J. Virol. 76 (3): 12233 - 12241. |
| Description of Target |
CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, gamma, delta, zeta) that assemble and function as three pairs of dimers (epsilon-gamma, epsilon-delta, zeta-zeta). The CD3 complex serves as a T-cell co-receptor that associates non-covalently with the T-cell receptor (TCR). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as a T-cell marker. |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for Rat anti-Human CD3 Antibody (OASA11552) |
| Specificity |
Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3-epilson chain recognizes human CD3-epsilon. As this product has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. |
| Additional Information |
Histology Positive Control Tissue: Tonsil
Carrier Free: Yes |
| Application Info |
FC: 1/50-1/100. Membrane permeabilization is required for this application
IHC-F: 1/100
IHC-P: 1/100. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer (pH 9.0) is recommended for this purpose. |
| Immunogen |
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC ) |
| Uniprot ID |
P07766 |
| Protein Name |
T-cell surface glycoprotein CD3 epsilon chain |
| Publications |
Lack of Major Histocompatibility Complex Class I Upregulation and Restrictive Infection by JC Virus Hamper Detection of Neurons by T Lymphocytes in the Central Nervous System. J Neuropathol Exp Neurol. 74, 791-803 (2015). 26115192
Natalizumab-associated progressive multifocal leukoencephalopathy in a patient with multiple sclerosis: a postmortem study. J Neuropathol Exp Neurol. 72, 1043-51 (2013). 24128680 |
| Protein Accession # |
NP_000724.1 |
| Purification |
Purified IgG prepared from tissue culture supernatant |
| Predicted Species Reactivity |
Human |
| Application |
Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin |
| Predicted Homology Based on Immunogen Sequence |
Bovine, dog, horse, Rhesus monkey, pig, chicken, mouse, duck, koala, Harbour porpoise, alpaca, Cynomolgus monkey, spotted hyena, sea lion, cat, Amazon parrot, raccoon, great horned-owl, bullfrog, xenopus, rabbit. Based on sequence similarity, is expected to react with: mammals, birds, amphibia. Antibody reactivity and working conditions may vary between species. Antibody reactivity and working conditions may vary between species. |
| Image 1 | |
