NME1 Antibody - N-terminal region (AVARP00009_P050)

Catalog No: AVARP00009_P050

NME1 antibody - N-terminal region (AVARP00009_P050) validated by WB using Fetal Heart Lysate at 1ug/ml.

Lanes:
1. 20 ug murine 4T1 primary breast tumor cell lysate
2. 20 ug human MDA-MB-231 cell lysate
3. 20 ug murine 4T1 primary breast tumor cell lysate
Primary Antibody Dilution:
1:4000
Secondary Antibody:
Goat anti-rabbit-Alexa Fluor 680
Secondary Antibody Dilution:
1:25,000
Gene Name:
NME1
Submitted by:
Anonymous

Lanes:
1. 20 ug murine 4T1 primary breast tumor cell lysate
2. 20 ug human MDA-MB-231 cell lysate
3. 20 ug murine 4T1 primary breast tumor cell lysate
Primary Antibody Dilution:
1:2000
Secondary Antibody:
Goat anti-rabbit-Alexa Fluor 680
Secondary Antibody Dilution:
1:25,000
Gene Name:
NME1
Submitted by:
Anonymous

Anti-NME1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

Anti-NME1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.

Human kidney

DB Suggested Anti-Nme1 antibody
Titration: 0.5 ug/ml
Positive Control: Recomninant human NME2 protein

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: DB, IHC, WB
• Additional Information: IHC Information: Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Nm23-H1 antibody (AVARP00009_P050).
  IHC Information: Pancreas
  IHC Information: Small intestine
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NME1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEH
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-NME1 (AVARP00009_P050) antibody is Catalog # AAP30457 (Previous Catalog # AAPP01041)
• Other Applications Image 1 Data: DB Suggested Anti-Nme1 antibody
  Titration: 0.5 ug/ml
  Positive Control: Recomninant human NME2 protein
  

Target Info

• Reference: Liu,S.J., (2008) Br. J. Cancer 98 (2), 363-369
• Gene Symbol: NME1
• Gene Full Name: Non-metastatic cells 1, protein (NM23A) expressed in
• Alias Symbols: NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1
• NCBI Gene Id: 4830
• Protein Name: Nucleoside diphosphate kinase B
• Description of Target: This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
• Uniprot ID: P22392
• Protein Accession #: NP_000260
• Nucleotide Accession #: NM_000269
• Protein Size (# AA): 152
• Molecular Weight: 17kDa
• Protein Interactions: SREK1IP1; PID1; TNPO2; POLR1C; NME4; NME3; NME1; TUBG1; UBC; MDM2; ASB18; BMI1; CHRAC1; NME1-NME2; WDR1; THOP1; SORD; RBBP7; OXCT1; IDH1; FDPS; MAPK14; FBXO6; WDYHV1; NIF3L1; SOX30; FXR2; SMARCD1; NME2; CCND3; UROD; SOD1; MIF; ERG; EBNA-LP; HDAC5; STRAP;