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NME1 ELISA Kit (Human) : 96 Wells (OKEH02350)

96 Wells
$599.00
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Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
NME1
Official Gene Full Name:
NME/NM23 nucleoside diphosphate kinase 1
Alias Symbols:
AWD, GAAD, Granzyme A-activated DNase, Metastasis inhibition factor nm23, NB, NBS, NDKA, NDK A, NDPKA, NDPK-A, NDP kinase A, NM23, nm23-H1, NM23-H1, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein
NCBI Gene Id:
4830
Protein Name:
Nucleoside diphosphate kinase A
Description of Target:
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Swissprot Id:
P15531
Protein Accession #:
NP_000260.1
Nucleotide Accession #:
NM_000269.2
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.056 ng/mL
Kit Range:
0.156 - 10ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology NME1 ELISA Kit (Human) (OKEH02350) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NME1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NME1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NME1 captured in well.
Kit Component:
ComponentAmount
NME1 Microplate96 Wells (12 x 8 Well strips)
NME1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated IKZF1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NME1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NME1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Nucleoside diphosphate kinase A
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
17 kDa
Protein Size (# AA):
152

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