NME1 ELISA Kit (Human) : 96 Wells (OKEH02350)

Catalog No: OKEH02350

Size:96T

Human NME1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NME1 ELISA Kit (Human) (OKEH02350) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NME1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NME1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NME1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.6% (n = 20)
  Mean Inter-assay CV%: < 7.7% (n = 20)
• ELISA Kit Component:
  • NME1 Microplate: 96 Wells (12 x 8 Well strips)
  • NME1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IKZF1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.056 ng/mL
• Specificity: Natural and recombinant Human Nucleoside diphosphate kinase A
• Publications:

  Zhenhua Du, et al. Quantitative proteomics identifies a plasma multi-protein model for detection of hepatocellular carcinoma. 15552 (2020). 32968147

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for NME1 ELISA Kit (Human) : 96 Wells (OKEH02350) on CiteAb

Target Info

• Gene Symbol: NME1
• Gene Full Name: NME/NM23 nucleoside diphosphate kinase 1
• Alias Symbols: AWD;epididymis secretory sperm binding protein;GAAD;granzyme A-activated DNase;metastasis inhibition factor nm23;NB;NBS;NDKA;NDP kinase A;NDPKA;NDPK-A;NM23;NM23-H1;non-metastatic cells 1, protein (NM23A) expressed in;nucleoside diphosphate kinase A;tumor metastatic process-associated protein.
• NCBI Gene Id: 4830
• Protein Name: P15531
• Description of Target: Nucleoside diphosphate kinase A
• Uniprot ID: https://www.uniprot.org/uniprot/P15531
• Protein Accession #: NP_000260.1
• Nucleotide Accession #: NM_000269.2
• Protein Size (# AA): 152
• Molecular Weight: 17 kDa