NME1 Antibody - N-terminal region (AVARP00009_P050)

• Product Number: AVARP00009_P050
• Name: NME1 Antibody - N-terminal region (AVARP00009_P050)
• Protein Size (# AA): 152 amino acids
• Molecular Weight: 17kDa
• NCBI Gene Id: 4830
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Non-metastatic cells 1, protein (NM23A) expressed in
• Alias Symbols: NB, AWD, NBS, GAAD, NDKA, NM23, NDPKA, NDPK-A, NM23-H1
• Peptide Sequence: Synthetic peptide located within the following region: ANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEH
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Liu,S.J., (2008) Br. J. Cancer 98 (2), 363-369
• Description of Target: This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
• Protein Interactions: SREK1IP1; PID1; TNPO2; POLR1C; NME4; NME3; NME1; TUBG1; UBC; MDM2; ASB18; BMI1; CHRAC1; NME1-NME2; WDR1; THOP1; SORD; RBBP7; OXCT1; IDH1; FDPS; MAPK14; FBXO6; WDYHV1; NIF3L1; SOX30; FXR2; SMARCD1; NME2; CCND3; UROD; SOD1; MIF; ERG; EBNA-LP; HDAC5; STRAP;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Nm23-H1 antibody (AVARP00009_P050).
  IHC Information: Pancreas
  IHC Information: Small intestine
• Other Applications Image 1 Data: DB Suggested Anti-Nme1 antibody
  Titration: 0.5 ug/ml
  Positive Control: Recomninant human NME2 protein
  
• Blocking Peptide: For anti-NME1 (AVARP00009_P050) antibody is Catalog # AAP30457 (Previous Catalog # AAPP01041)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NME1
• Uniprot ID: P22392
• Protein Name: Nucleoside diphosphate kinase B
• Protein Accession #: NP_000260
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000269
• Tested Species Reactivity: Human
• Gene Symbol: NME1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit
• Application: DB, IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Fetal heart
  
  NME1 antibody - N-terminal region (AVARP00009_P050) validated by WB using Fetal Heart Lysate at 1ug/ml.
• Image 2: Mouse, Human
  
  Lanes:
  1. 20 ug murine 4T1 primary breast tumor cell lysate
  2. 20 ug human MDA-MB-231 cell lysate
  3. 20 ug murine 4T1 primary breast tumor cell lysate
  Primary Antibody Dilution:
  1:4000
  Secondary Antibody:
  Goat anti-rabbit-Alexa Fluor 680
  Secondary Antibody Dilution:
  1:25,000
  Gene Name:
  NME1
  Submitted by:
  Anonymous
  
• Image 3: Mouse, Human
  
  Lanes:
  1. 20 ug murine 4T1 primary breast tumor cell lysate
  2. 20 ug human MDA-MB-231 cell lysate
  3. 20 ug murine 4T1 primary breast tumor cell lysate
  Primary Antibody Dilution:
  1:2000
  Secondary Antibody:
  Goat anti-rabbit-Alexa Fluor 680
  Secondary Antibody Dilution:
  1:25,000
  Gene Name:
  NME1
  Submitted by:
  Anonymous
  
• Image 4: human NME2
  
  DB Suggested Anti-Nme1 antibody
  Titration: 0.5 ug/ml
  Positive Control: Recomninant human NME2 protein
• Image 5: Human kidney
  
  Human kidney
• Image 6: Human small intestine
  
  Anti-NME1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.
• Image 7: Human pancreas
  
  Anti-NME1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Primary Antibody concentration 5 ug/ml.