KIT Antibody : APC (OATA00068)

Catalog#: OATA00068

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: ACK2
• Isotype: Rat IgG2b, kappa
• Host: Rat
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Gene Symbol: KIT
• NCBI Gene Id: 16590
• Official Gene Full Name: KIT proto-oncogene receptor tyrosine kinase
• Protein Name: mast/stem cell growth factor receptor Kit
• Swissprot Id: P05532
• Protein Accession #: NP_001116205.1
• Nucleotide Accession #: NM_001122733.1
• Alias Symbols: W, Bs, Fdc, Ssm, SCO1, SCO5, SOW3, CD117, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3
• Description of Target: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express KIT.
• Specificity: CD117 (c-Kit)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Target Reference:

  Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)

  Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)

  Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot - Fischer 344 Rat)

  Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)

  Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D"™Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)

  Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)

  Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry - paraffin embedded tissue, in vivo blocking)

Protocol:

Tips Information: