KIT Antibody : APC (OATA00068)

Data Sheet
 
Product Number OATA00068
Product Page www.avivasysbio.com/kit-antibody-apc-oata00068.html
Name KIT Antibody : APC (OATA00068)
Clone ACK2
Isotype Rat IgG2b, kappa
Conjugation APC: Allophycocyanin
NCBI Gene Id 16590
Host Rat
Clonality Monoclonal
Gene Full Name KIT proto-oncogene receptor tyrosine kinase
Alias Symbols W, Bs, SC, SO, Fdc, Ssm, Gsfs, SCO1, SCO5, SOW3, Tr-k, c-KI, CD117, belly, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3
Product Format Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Reference

Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)

Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)

Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot - Fischer 344 Rat)

Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)

Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D"™Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)

Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)

Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry - paraffin embedded tissue, in vivo blocking)

Description of Target The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Reconstitution and Storage 2-8C protected from light
Datasheets/Manuals Printable datasheet for OATA00068
Specificity CD117 (c-Kit)
Additional Information Excitation Laser: Red (633-640 nm)
Application Info This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
Additional Information Emmision: 660 nm
Uniprot ID P05532
Protein Name mast/stem cell growth factor receptor Kit
Protein Accession # NP_001116205.1?
Nucleotide Accession # NM_001122733.1
Tested Species Reactivity Mouse
Gene Symbol KIT
Application FC
Image 1
C57Bl/6 bone marrow cells
C57Bl/6 bone marrow cells were stained with FITC Anti-Mouse CD45R (B220) (OATA00213) and 0.06 ug APC Anti-Mouse CD117 (OATA00068) (right panel) or 0.06 ug APC Rat IgG2b (left panel).
 

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