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Human CD25 Antibody (OATA00537)

100 ug
$51.00
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Tested Species Reactivity:
Human
Predicted Species Reactivity:
Baboon, Chimpanzee, Cynomolgus, Rhesus
Product Format:
Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Clonality:
Monoclonal
Clone:
BC96
Isotype:
Mouse IgG1, kappa
Host:
Mouse
Conjugation:
Unconjugated
Application:
FC
Additional Information:
Description:

The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.

The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on -induced- peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.

Reconstitution and Storage:
2-8C
Datasheets/Manuals:
Printable datasheet for OATA00537
Specificity:
CD25
Application Info:
This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Citations are provided as a convenience to you - please consult Materials and Methods sections for additional details about the use of any product in these publications.
Target Reference:

Willer DO, Ambagala APN, Pilon R, chan JK, Fournier J, Brooks J, Sandstrom P, and MacDonald KS. 2012. J. Virol. 86: 3626-3634. (Flow cytometry - cynomolgus)

Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)

Mackroth MS, Malhotra I, Mungai P, Koech D, Muchiri E, and King CL. 2011. J. Immunol. 186: 2780-2791. (Flow cytometry)

Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y, and Yoshimura A. 2011. Int. Immunol. 23(11):701-712. (Cell sorting / negative selection - T cells)

Wan Q, Kozhaya L, ElHed A, Ramesh R, Carlson TJ, Djretic IM, Sundrud MS, and Unutmax D. 2011. J. Exp. Med. 208: 1875-1887. (Cell sorting / negative selection - T cells)

Verhoeven D, Sankaran S, Silvey M, and Dandekar S. 2008. J. Virol. 82: 4016-4027. (Flow cytometry - rhesus macaque)

Gene Symbol:
IL2RA
Official Gene Full Name:
interleukin 2 receptor subunit alpha
Alias Symbols:
p55, CD25, IL2R, IMD41, TCGFR, IDDM10
NCBI Gene Id:
3559
Protein Name:
interleukin-2 receptor subunit alpha
Description of Target:
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Swissprot Id:
P01589
Protein Accession #:
NP_000408.1
Nucleotide Accession #:
NM_000417.2
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL2RA.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL2RA.

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