FHIT Protein (OPPA01214)

Catalog No: OPPA01214 (Formerly GWB-B45B78)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: FHIT in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol. Physical appearance: Sterile Filtered clear solution.
• Host: E. Coli
• Application: ELISA, NT, WB
  Product Introduction: FHIT is a member of the histidine triad gene family. FHIT gene encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. FHIT gene includes the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and abnormal transcripts of this gene. The FHIT protein is a tumor suppressor with reduced or no expression in numerous types of cancer. FHIT may also act as a tumor suppressor in normal cells. Alterations and deletions of the FHIT (Fragile Histidine Triad) gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. The expression of FHIT is more often lost in cancers of individuals with familial mutations causing deficiency in DNA repair genes such as BRCA1, BRCA2 and MSH2. In vitro FHIT functions as a hydrolase that cleaves diadenosine triphosphate (Ap3A) to ADP and AMP. The FHIT -Ap3A enzyme-substrate complex seems to be the tumor suppressor signal. The restoration of FHIT expression in FHIT -deficient cancer cells leads to apoptosis, involving the intrinsic caspase pathway, in cancer-derived cells and in tumor xenografts.
  Product Description: FHIT Human Recombinant full length protein expressed in E.coli, shows a 43 kDa band on SDS-PAGE. The FHIT is fused to GST-Tag and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store vial at -20C to -80C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

Target Info

• Gene Symbol: FHIT
• Alias Symbols: FRA3B, AP3Aase
• NCBI Gene Id: 2272
• Protein Name: Bis(5'-adenosyl)-triphosphatase
• Description of Target: Recombinant Human Fragile Histidine Triad, GST Tag
• Uniprot ID: P49789
• Protein Accession #: NP_001159715.1
• Protein Size (# AA): Recombinant