FHIT Antibody - middle region (OASG02715)

Catalog No: OASG02715

Size:100 uL

Western Blot using A549

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF, ELISA
• Additional Information: Cellular Localization: Cytoplasm. Mitochondrion. Nucleus.
  Tissue Specificity: Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the Internal region of human Fhit. Location: 60-140aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Specificity: Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.
• Application Info: WB: 1:500~2000
  IHC: 1:100~300
  IF: 1:200~1000
  ELISA: 1:10000

Target Info

• Gene Symbol: FHIT
• Gene Full Name: fragile histidine triad
• Alias Symbols: FRA3B, AP3Aase
• NCBI Gene Id: 2272;
• Description of Target: Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
• Uniprot ID: P49789
• Molecular Weight: 16 kDa