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| Datasheets/Manuals | Printable datasheet for COL1A1 Antibody - C-terminal region (OABB01300) |
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| Tested Species Reactivity | Human, Mouse, Rat |
|---|---|
| Predicted Species Reactivity | Hamster|Human|Mouse|Rat |
| Product Format | Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
| Clonality | Polyclonal |
| Clone | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot |
| Additional Information | Notes: WB: The detection limit for COL1A1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
| :: | Background: Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Polypeptide |
| Purification | Affinity Purified |
| Concentration | 500 ug/ml |
| Specificity | No cross reactivity with other proteins. |
| Application Info | Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human, Rat: Mouse Immunocytochemistry : 0.5-1 ug/ml: Mouse: Human, Rat Immunofluorescence: 2 ug/ml: Human |
| Storage Buffer | Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4. |
| Description | Rabbit IgG polyclonal antibody for Collagen alpha-1(I) chain(COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC, IF in Human;Mouse;Rat. |
| Gene Symbol | Col1a1 |
|---|---|
| Gene Full Name | collagen, type I, alpha 1 |
| Alias Symbols | alpha-1 type 1 collagen;alpha-1 type I collagen;Co;Col;Col1;Col1a-1;Cola1;Cola-1;collagen alpha-1(I) chain;Mov-;Mov13;Mov-13;procollagen, type I, alpha 1. |
| NCBI Gene Id | 12842 |
| Protein Name | Collagen alpha-1(I) chain |
| Description of Target | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
| Uniprot ID | P11087 |
| Molecular Weight | 138032 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "COL1A1 Antibody - C-terminal region (OABB01300)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Hamster|Human|Mouse|Rat".
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How long will it take to receive "COL1A1 Antibody - C-terminal region (OABB01300)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "COL1A1 Antibody - C-terminal region (OABB01300)" provided in?
This item is provided in "Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "COL1A1 Antibody - C-terminal region (OABB01300)"?
This target may also be called "alpha-1 type 1 collagen;alpha-1 type I collagen;Co;Col;Col1;Col1a-1;Cola1;Cola-1;collagen alpha-1(I) chain;Mov-;Mov13;Mov-13;procollagen, type I, alpha 1." in publications.
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What is the shipping cost for "COL1A1 Antibody - C-terminal region (OABB01300)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "COL1A1 Antibody - C-terminal region (OABB01300)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "COL1A1 Antibody - C-terminal region (OABB01300)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "138032 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "COL1A1 Antibody - C-terminal region (OABB01300)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Col1a1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Col1a1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Col1a1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Col1a1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Col1a1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Col1a1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
