COL1A1 Antibody - C-terminal region (OAAB10798)

Catalog No: OAAB10798

Size:400ul

COL1A1 Antibody (C-term) (Cat. #OAAB10798) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the COL1A1 antibody detected the COL1A1 protein (arrow).

COL1A1 Antibody (C-term) (Cat. #OAAB10798)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Function: Type I collagen is a member of group I collagen (fibrillar forming collagen).
  Cellular Location: Secreted, extracellular space, extracellular matrix (By similarity).
  Tissue Location: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This COL1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1077-1106 amino acids from the C-terminal region of human COL1A1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine; Chicken; Rat
• Concentration: 0.5 mg/ml (Actual concentration varies with each lot)
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Publications:

  Discoidin domain receptor 1 activation links extracellular matrix to podocyte lipotoxicity in Alport syndrome. EBioMedicine. 63, 103162 (2020). 33340991

  Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts. J Clin Invest. 131 (2021). 33465051

Target Info

• Gene Symbol: COL1A1
• Alias Symbols: OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1
• NCBI Gene Id: 1277
• Protein Name: Collagen alpha-1(I) chain
• Description of Target: This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish].
• Uniprot ID: P02452
• Protein Accession #: NP_000079.2
• Molecular Weight: 139 kDa