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COL1A1 Antibody - C-terminal region (ARP59998_P050)

100 ul
$319.00
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Conjugation Options

ARP59998_P050-FITC Conjugated

ARP59998_P050-HRP Conjugated

ARP59998_P050-Biotin Conjugated

Tested Species Reactivity:
Human
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Clonality:
Polyclonal
Host:
Rabbit
Application:
WB
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
COL1A1
Official Gene Full Name:
Collagen, type I, alpha 1
NCBI Gene Id:
1277
Protein Name:
Collagen alpha-1(I) chain
Swissprot Id:
P02452
Protein Accession #:
NP_000079
Nucleotide Accession #:
NM_000088
Alias Symbols:
OI4
Replacement Item:
This antibody may replace item sc-125157 from Santa Cruz Biotechnology.
Description of Target:
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Protein Size (# AA):
1464
Molecular Weight:
137kDa
Purification:
Affinity Purified
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express COL1A1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express COL1A1.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human COL1A1
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
Complete computational species homology data:
Anti-COL1A1 (ARP59998_P050)
Peptide Sequence:
Synthetic peptide located within the following region: YRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMC
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
PDGFB; PDGFA; COL1A2; COL1A1; CAPN1; UBC; BRCA1; BARD1; C12orf57; RNH1; ATP13A2; ELAVL1; ACHE; TMPRSS6; P4HB; PAK1; HTRA1; MATN2; PRELP; TGFBI; BGN; VWF; ITGA2; DDR2; THBS1; TXN; SPARC; CD36; FGF7; IGFBP3; ITGA5; FN1; NID1; DCN; CD93; MMP9; MMP2; COL7A1;
Blocking Peptide:
For anti-COL1A1 (ARP59998_P050) antibody is Catalog # AAP59998 (Previous Catalog # AAPP46152)
Datasheets/Manuals:
Printable datasheet for anti-COL1A1 (ARP59998_P050) antibody

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1 Item(s)

78/03/2019 03:15
  • Overall Experience:
  • Quality:
Primary human lung fibroblasts in WB

Submitted by:
Thomas Thatcher, Ph.D.
University of Rochester

COL1A1

1. Sample type: Primary human lung fibroblasts treated with TGFB for 3 days. Primary human lung fibroblasts treated with TGFB to induce collagen expression and transfected with siRNA.

2. Primary antibody concentration: 1ug/ml. Overnight 4˚ in 5% NFDM

3. Secondary antibody and dilution: Goat anti-Rabbit. 1:2000, 1:5000 in 5% NFDM for 1 hr at room temp.

4. Protocol:
1. PVDF membrane
2. Blocked in 5% NFDM in TBS-T for 30 minutes

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