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Anti-Collagen Type I Antibody (OARA02579)

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Gene Symbol:
NCBI Gene Id:
Swissprot Id:
Alias Symbols:
Collagen Of Skin Tendon And Bone, Collagen Type 1,Collagen type I alpha 1, Collagen type I alpha 2, OI4, Osteogenesis Imperfecta Type IV, COL1A1
Replacement Item:
This antibody may replace item sc-125157 from Santa Cruz Biotechnology.
Description of Target:
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Collagen Type I is a protein that strengthens and supports many tissues in the body, including cartilage, bone, tendon, skin and the white part of the eye (sclera). Collagen Type I triple helix comprises of two alpha1 chains and one alpha2 chain. COL1A1/A2 could be useful for detecting melanoma, lung, liver, glioma, skin, stomach, and other cancers. Mutations in the gene may be related to caffey disease, osteogenesis, and ehlers-danlos syndrome. Anti-Collagen Type I Antibody is ideal for investigators involved in extracellular matrix protein, osteoporosis research, Cell Biology, Signal Transduction, and Stem Cell research.
Anti-Collagen Type I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Collagen Type I from human and bovine placenta.
Immunogen type:Native Protein
Species Reactivity:
Human, Bovine
Product Format:
Liquid 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.0005M EDTA (pH 8.0) with 0.01% (w/v) Sodium Azide
Reconstitution and Storage:
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
1.0 mg/ml (by UV absorbance at 280 nm)
Printable datasheet for OARA02579
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Application Info:
ELISA: 1:5000~50000
FLISA: 1:100
IHC: 1:50~200
IP: 1:100
WB: 1:1000~10000
Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

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