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Datasheets/Manuals | Printable datasheet for Anti-Collagen Type I Antibody (OARA02579) |
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Predicted Species Reactivity | Human, Mouse, Rat, Bovine, Pig |
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Product Format | Liquid 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Dot blot|Enzyme-linked immunosorbent assay|Flow cytometry|Fluorescence-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunoprecipitation|Multiplex assay|Western blot |
Reconstitution and Storage | Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Immunogen | Collagen Type I from human and bovine placenta |
Purification | COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens. |
Concentration | Varies by lot. See vial for concentration. |
Specificity | Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
Dilution | ELISA: 1:5000~50000 FLISA: 1:100 IHC: 1:50~200 IP: 1:100 |
Application Info | Anti-Collagen Type I has been tested by Western blot, dot blot, and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, IP, native PAGE, immunofluorescence, and FC for highly sensitive qualitative analysis. |
Gene Symbol | COL1A1 |
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Gene Full Name | collagen type I alpha 1 chain |
Alias Symbols | alpha-1 type I collagen;alpha1(I) procollagen;CAFYD;collagen alpha 1 chain type I;collagen alpha-1(I) chain;collagen alpha-1(I) chain preproprotein;collagen of skin, tendon and bone, alpha-1 chain;collagen, type I, alpha 1;EDSARTH1;EDSC;OI1;OI2;OI3;OI4;pro-alpha-1 collagen type 1;type I proalpha 1;type I procollagen alpha 1 chain. |
NCBI Gene Id | 1277 |
Protein Name | Collagen alpha-1(I) chain|collagen alpha-1(I) chain preproprotein [Homo sapiens] |
Description of Target | Type I collagen is a member of group I collagen (fibrillar forming collagen). |
Uniprot ID | P02452 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_000079.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Collagen Type I Antibody (OARA02579)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Pig".
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How long will it take to receive "Anti-Collagen Type I Antibody (OARA02579)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Anti-Collagen Type I Antibody (OARA02579)" provided in?
This item is provided in "Liquid 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Collagen Type I Antibody (OARA02579)"?
This target may also be called "alpha-1 type I collagen;alpha1(I) procollagen;CAFYD;collagen alpha 1 chain type I;collagen alpha-1(I) chain;collagen alpha-1(I) chain preproprotein;collagen of skin, tendon and bone, alpha-1 chain;collagen, type I, alpha 1;EDSARTH1;EDSC;OI1;OI2;OI3;OI4;pro-alpha-1 collagen type 1;type I proalpha 1;type I procollagen alpha 1 chain." in publications.
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What is the shipping cost for "Anti-Collagen Type I Antibody (OARA02579)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Collagen Type I Antibody (OARA02579)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Collagen Type I Antibody (OARA02579)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Collagen Type I Antibody (OARA02579)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "COL1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "COL1A1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "COL1A1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "COL1A1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "COL1A1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.