Brevican Antibody (OASE00276)

Catalog No: OASE00276

Size:100ug

Western Blot analysis of Rat Brain Membrane showing detection of ~270 kDa Notch1 protein using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug).. Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~270 kDa.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Notch1 Monoclonal Antibody, Clone S253-32. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Notch1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Notch1 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S294A-6
• Isotype: IgG2B
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, ICC, IF
• Additional Information: Background Info: Detects ~140kDa (and smaller due to proteolytic cleavage)
  Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult
  brain. It is a member of the lectican family of
  aggregating extracellular matrix proteoglycans that bear
  chondroitin sulfate (CS) chains. It is highly expressed in
  the central nervous system and is thought to stabilize
  synapses and inhibit neural plasticity. Brevican is
  secreted from astrocytes and neurons as a 145 kD core
  protein that bears up to three, covalently-linked, CS
  chains. It is also is secreted as a 145 kD core protein
  without CS chains. When cleaved by extracellular
  glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Brevican in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 219-655 of rat Brevican
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~140kDa (and smaller due to proteolytic cleavage).
• Dilution: WB (1:1000); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126.
  2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M.
  and Yamaguchi Y. (1995) Biochem. Biophys. Res.
  Commun. 216: 957-963.
  3. Rauch U., et al. (1997) Genomics 44: 15-21.
  4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and
  Hockfield S. (1998) J. Neurosci. 7: 2370-2376.
  5. Aspberg A., Adam S., Kostka G., Timpl R. and
  Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449.
• Description: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.

Target Info

• Gene Symbol: Brevican
• Gene Full Name: brevican
• Alias Symbols: BCAN, BEHAB, CSPG7, Brain enriched hyaluronan binding protein, Brevican core protein, Brevican core protein isoform 1, Brevican core protein isoform 2, Brevican proteoglycan, Chondroitin sulfate proteoglycan 7, Chondroitin sulfate proteoglycan BEHAB, MGC13038
• NCBI Gene Id: 25393
• Uniprot ID: P55068
• Protein Accession #: NP_001028837.1