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Datasheets/Manuals | Printable datasheet for OASE00276-HRP |
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Predicted Species Reactivity | Mouse, Rat |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | S294A-6 |
Isotype | IgG2B |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | ICC, WB |
Additional Information | Background Info: Detects ~140kDa (and smaller due to proteolytic cleavage) |
:: | Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE. |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of Brevican in 20ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Fusion protein amino acids 219-655 of rat Brevican, accession number P55068. |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:1000 WB |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Yamada H., Watanabe K., Shimonaka M. and Yamaguchi Y. (1994) J. Biol. Chem. 269: 10119-10126. 2. Yamada H., Watanabe K., Shimonaka M., Yamasaki M. and Yamaguchi Y. (1995) Biochem. Biophys. Res. Commun. 216: 957-963. 3. Rauch U., et al. (1997) Genomics 44: 15-21. 4. Zhang H., Kelly G., Zerillo C., Jaworski D.M. and Hockfield S. (1998) J. Neurosci. 7: 2370-2376. 5. Aspberg A., Adam S., Kostka G., Timpl R. and Heinegard D. (1999) J. Biol. Chem. 274: 20444-20449. |
Gene Symbol | Bcan |
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Gene Full Name | brevican |
Alias Symbols | BEHAB, ALPBRE |
NCBI Gene Id | 25393 |
Uniprot ID | P55068 |
Protein Accession # | NP_001028837.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Brevican Antibody : HRP (OASE00276-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat".
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How long will it take to receive "Brevican Antibody : HRP (OASE00276-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Brevican Antibody : HRP (OASE00276-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Brevican Antibody : HRP (OASE00276-HRP)"?
This target may also be called "BEHAB, ALPBRE" in publications.
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What is the shipping cost for "Brevican Antibody : HRP (OASE00276-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Brevican Antibody : HRP (OASE00276-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Brevican Antibody : HRP (OASE00276-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Brevican Antibody : HRP (OASE00276-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BCAN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BCAN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BCAN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BCAN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BCAN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BCAN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.