Villin Antibody (OASL01258)

Catalog No: OASL01258

Size:50ul

Immunohistochemical analysis of paraffin-embedded Appendix. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval.

Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval.

Product Info

• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Monoclonal
• Isotype: IgG2b, Kappa
• Host: Mouse
• Application: IHC-P
• Additional Information: Background: villin 1(VIL1) Homo sapiens this gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.
  Function: domain: consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium, Ca(2+)-regulated actin-binding protein, similarity: belongs to the villin/gelsolin family, similarity: contains 1 HP (headpiece) domains, similarity: contains 6 gelsolin-like repeats, subunit: Monomer, tissue specificity: Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  Location: Membranous, Cytoplasmic.
  Expression: Intestine, Kidney, Liver.
• Reconstitution and Storage: Store at -20C for one year. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human Villin
• Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Concentration: 1 mg/ml
• Specificity: This antibody detects endogenous levels of human Villin
• Application Info: IHC-P 1:200

Target Info

• Gene Symbol: VIL1
• Alias Symbols: VIL, D2S1471
• NCBI Gene Id: 7429
• Protein Name: Villin-1
• Uniprot ID: P09327