VAChT Antibody (OASE00241)

Catalog No: OASE00241

Size:100ug

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: S6-38
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF
• Additional Information: Background Info: No known cross-over to other proteins
  Scientific Background: VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
  Certificate of Analysis: A dilution of 1:50-1:200 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Synthetic peptide amino acids 521-532 of human VAChT
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~56kDa.
• Dilution: WB (1:1000), IHC (1:200), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Erickson J.D., Varoqui H. (2000) FASEB J. 14(15): 2450-2458. 2.     Weihe E., Tao-Cheng J.H., Schafer M.K., Erickson J.D., Eiden L.E. (1996) Proc Natl Acad Sci USA. 93(8): 3547-3552.
• Description: VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

Target Info

• Gene Symbol: VAChT
• Gene Full Name: solute carrier family 18 (vesicular acetylcholine transporter), member 3
• Alias Symbols: Vesicular Acetylcholine Transporter, MGC12716, rVAT, Slc18a3, Solute carrier family 18 (vesicular acetylcholine) member 3, Solute carrier family 18 (vesicular monoamine) member 3, Solute carrier family 18 member 3
• NCBI Gene Id: 6572
• Uniprot ID: Q16572
• Protein Accession #: NP_003046.2