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Datasheets/Manuals | Printable datasheet for anti-UBE3A (ARP42950_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human UBE3A |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 93%; Goat: 90%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Yeast: 91%; Zebrafish: 100% |
Peptide Sequence | Synthetic peptide located within the following region: SLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGP |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-UBE3A (ARP42950_P050) antibody is Catalog # AAP42950 (Previous Catalog # AAPS13301) |
Sample Type Confirmation | UBE3A is strongly supported by BioGPS gene expression data to be expressed in HeLa |
Reference | Bedard,K.M., (2008) J. Virol. 82 (8), 3894-3902 |
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Gene Symbol | UBE3A |
Gene Full Name | Ubiquitin protein ligase E3A |
Alias Symbols | AS, ANCR, E6-AP, HPVE6A, EPVE6AP |
NCBI Gene Id | 7337 |
Protein Name | Ubiquitin-protein ligase E3A |
Description of Target | UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. |
Uniprot ID | Q05086 |
Protein Accession # | NP_000453 |
Nucleotide Accession # | NM_000462 |
Protein Size (# AA) | 875 |
Molecular Weight | 101kDa |
Protein Interactions | KLHL38; UBE2D1; UBB; TP53; TAT; AFG3L1P; ATG9A; ASAP3; AHSP; UBE3A; UBC; MEOX2; MAGEA8; JADE1; YOD1; UBE2L3; UBE2D3; PSMD14; SHFM1; ARNTL; LPP; HSF1; DR1; BTF3L4; LRSAM1; P3H1; NPLOC4; IPO11; DBNL; LSM1; ARPC1B; METTL1; SACS; ARC; RAD23A; PTPN3; CEBPA; SC |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish".
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How long will it take to receive "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "UBE3A Antibody - N-terminal region (ARP42950_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
This target may also be called "AS, ANCR, E6-AP, HPVE6A, EPVE6AP" in publications.
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What is the shipping cost for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "101kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UBE3A Antibody - N-terminal region (ARP42950_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "UBE3A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "UBE3A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "UBE3A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "UBE3A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.