UBE3A Antibody - N-terminal region : HRP (ARP42950_P050-HRP)

Catalog No: ARP42950_P050-HRP

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE3A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Goat: 90%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Yeast: 91%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-UBE3A (ARP42950_P050-HRP) antibody is Catalog # AAP42950 (Previous Catalog # AAPS13301)
• Sample Type Confirmation:

  UBE3A is strongly supported by BioGPS gene expression data to be expressed in HeLa

Target Info

• Reference: Bedard,K.M., (2008) J. Virol. 82 (8), 3894-3902
• Gene Symbol: UBE3A
• Gene Full Name: Ubiquitin protein ligase E3A
• Alias Symbols: AS, ANCR, E6-AP, HPVE6A, EPVE6AP
• NCBI Gene Id: 7337
• Protein Name: Ubiquitin-protein ligase E3A
• Description of Target: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
• Uniprot ID: Q05086
• Protein Accession #: NP_000453
• Nucleotide Accession #: NM_000462
• Protein Size (# AA): 875
• Molecular Weight: 101kDa
• Protein Interactions: KLHL38; UBE2D1; UBB; TP53; TAT; AFG3L1P; ATG9A; ASAP3; AHSP; UBE3A; UBC; MEOX2; MAGEA8; JADE1; YOD1; UBE2L3; UBE2D3; PSMD14; SHFM1; ARNTL; LPP; HSF1; DR1; BTF3L4; LRSAM1; P3H1; NPLOC4; IPO11; DBNL; LSM1; ARPC1B; METTL1; SACS; ARC; RAD23A; PTPN3; CEBPA; SC