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| Datasheets/Manuals | Printable datasheet for UBE3A Antibody (OACD08106) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Range: Ser158 - Leu407 |
| Purification | Affinity Purified; Protein A Purified |
| Concentration | 0.5 mg/ml |
| Preservative | 0.02% NaN3 |
| Specificity | The antibody is a rabbit polyclonal antibody raised against UBE3A. It has been selected for its ability to recognize UBE3A in immunohistochemical staining and western blotting. |
| Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
| Gene Symbol | UBE3A |
|---|---|
| Gene Full Name | ubiquitin protein ligase E3A |
| Alias Symbols | ANCR;AS;CTCL tumor antigen se37-2;E6-AP;E6AP ubiquitin-protein ligase;EPVE6AP;HECT-type ubiquitin transferase E3A;HPVE6A;human papilloma virus E6-associated protein;human papillomavirus E6-associated protein;oncogenic protein-associated protein E6-AP;renal carcinoma antigen NY-REN-54;ubiquitin-protein ligase E3A. |
| NCBI Gene Id | 7337 |
| Protein Name | Ubiquitin-protein ligase E3A |
| Description of Target | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates (PubMed:10373495, PubMed:16772533, PubMed:19204938, PubMed:19233847, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24273172, PubMed:24728990). Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:10373495, PubMed:19204938, PubMed:19325566, PubMed:19591933, PubMed:22645313, PubMed:24728990). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins (PubMed:19233847). Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation (PubMed:16772533). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (By similarity). Synergizes with WBP2 in enhancing PGR activity (PubMed:16772533). |
| Uniprot ID | Q05086 |
| Protein Accession # | NP_000453.2 |
| Nucleotide Accession # | NM_000462 |
| Molecular Weight | 98 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UBE3A Antibody (OACD08106)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "UBE3A Antibody (OACD08106)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "UBE3A Antibody (OACD08106)" provided in?
This item is provided in "Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UBE3A Antibody (OACD08106)"?
This target may also be called "ANCR;AS;CTCL tumor antigen se37-2;E6-AP;E6AP ubiquitin-protein ligase;EPVE6AP;HECT-type ubiquitin transferase E3A;HPVE6A;human papilloma virus E6-associated protein;human papillomavirus E6-associated protein;oncogenic protein-associated protein E6-AP;renal carcinoma antigen NY-REN-54;ubiquitin-protein ligase E3A." in publications.
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What is the shipping cost for "UBE3A Antibody (OACD08106)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UBE3A Antibody (OACD08106)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UBE3A Antibody (OACD08106)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "98 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UBE3A Antibody (OACD08106)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "UBE3A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "UBE3A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "UBE3A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "UBE3A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
