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Datasheets/Manuals | Printable datasheet for OAAB15773 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC, IF, FC |
Additional Information | Cellular Location: Nucleus (Probable). |
Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This UBE3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 836-865 amino acids from the C-terminal region of human UBE3A. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Varies by lot. See vial for concentration. |
Application Info | IF: 1:10~50 WB: 1:1000 IHC-P: 1:50~100 FC: 1:10~50 |
Gene Symbol | UBE3A |
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Alias Symbols | AS, ANCR, E6-AP, HPVE6A, EPVE6AP |
NCBI Gene Id | 7337 |
Protein Name | Ubiquitin-protein ligase E3A |
Description of Target | UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain. |
Uniprot ID | Q05086 |
Protein Accession # | NP_000453.2 |
Molecular Weight | 101 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "UBE3A Antibody - C-terminal region (OAAB15773)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "UBE3A Antibody - C-terminal region (OAAB15773)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "UBE3A Antibody - C-terminal region (OAAB15773)" provided in?
This item is provided in "Liquid PBS with 0.09% (W/V) sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "UBE3A Antibody - C-terminal region (OAAB15773)"?
This target may also be called "AS, ANCR, E6-AP, HPVE6A, EPVE6AP" in publications.
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What is the shipping cost for "UBE3A Antibody - C-terminal region (OAAB15773)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "UBE3A Antibody - C-terminal region (OAAB15773)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "UBE3A Antibody - C-terminal region (OAAB15773)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "101 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "UBE3A Antibody - C-terminal region (OAAB15773)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "UBE3A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "UBE3A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "UBE3A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "UBE3A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "UBE3A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.