Human PTEN Antibody (Phospho-Ser380) (OAAQ00144)

Catalog No: OAAQ00144

Flow cytometric analysis of A431 cells, untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using Phospho-PTEN (S380) antibody, PTENS380-NA9 at 0.1 ug/mL, Cat. #OAAQ00144.

Peptide blocking flow cytometric analysis of A431 cells secondary antibody only negative control (light blue) or untreated (red) or treated with EGF (green) or untreated and blocked with phospho-peptide (black) or EGF and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or EGF and blocked with non-phospho peptide (purple) using Phospho-PTEN (S380) antibody PTENS380-NA9 0.05 ug/mL. Cat. #OAAQ00144.

PTENS380-NA9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or of Phospho-PTEN (S380) antibody PTENS380-NA9 (green) Cat. #OAAQ00144.

Peptide blocking flow cytometric analysis of A431 cells secondary antibody only negative control (light blue) or untreated (red) or treated with EGF (green) or untreated and blocked with phospho-peptide (black) or EGF and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or EGF and blocked with non-phospho peptide (purple) using Phospho-PTEN (S380) antibody PTENS380-NA9 0.05 ug/mL. Cat. #OAAQ00144.

Flow cytometric analysis of A431 cells, untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using Phospho-PTEN (S380) antibody, PTENS380-NA9 at 0.1 ug/mL, Cat. #OAAQ00144.

PTENS380-NA9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or of Phospho-PTEN (S380) antibody PTENS380-NA9 (green) Cat. #OAAQ00144.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: PTENS380-NA9
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human phospho PTEN
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Ser380
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: Li J, Yen C, Liaw D, et al. (1997) Science. 275:1943-1947.
  Maehama T, and Dixon JE. (1998) Journal of Biological Chemistry. 273:13375-13378.
  Zhang L, Zhang S, You J, et al. (2015) Nature. 527:100-104.
  Chen Z, Dempsey DR, Thomas SN, Hayward D, Bolduc DM, and Cole PA. (2016) Journal of Biological Chemistry. 291:14160-14169.

Target Info

• Gene Symbol: PTEN
• Gene Full Name: phosphatase and tensin homolog
• Alias Symbols: BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta
• NCBI Gene Id: 5728
• Protein Name: phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
• Description of Target: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
• Uniprot ID: P60484
• Protein Accession #: NP_000305.3
• Nucleotide Accession #: NM_000314.6