Human MAP3K5 Antibody : FITC (Phospho-Ser83) (OAAQ00084)

Catalog No: OAAQ00084

Flow cytometric analysis of HT1080 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Ask1 (Ser83) FITC-conjugated antibody Ask1S83-G4. Cat. #OAAQ00084.

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.09% NaN3, 0.2% BSA.
• Clonality: Monoclonal
• Clone: Ask1S83-G4
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: FC
• Reconstitution and Storage: 2-8C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Ser83
• Application Info: For flow cytometric staining, the suggested use of this reagent is 5 uL per million cells or 5 uL per 100 uL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
• Reference: Matsukawa J, et al., (2004) J Biochem 136: 261-265.
  Shizaka S., et al., 2013, Adv Biol Reg 53: 135-144.
  Sekine Y., et al., (2006), Curr Mol Medicine, 6: 87-89.
  Matsuzawa A., et al., (2002) Antioxidants Redox Signal 4:415-425.
  Nagai H., el al., (2007), BMB report, 40:1-6.
  Kulkarni S., et al., (2000), J Clin Invest 112:3555-3562.

Target Info

• Gene Symbol: MAP3K5
• Gene Full Name: mitogen-activated protein kinase kinase kinase 5
• Alias Symbols: ASK1, MEKK5, MAPKKK5
• NCBI Gene Id: 4217
• Protein Name: mitogen-activated protein kinase kinase kinase 5
• Description of Target: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
• Uniprot ID: Q99683
• Protein Accession #: NP_005914.1
• Nucleotide Accession #: NM_005923.3