Human MAP2K3 Antibody (Phospho-Ser189/Ser207) (OAAQ00145)

Catalog No: OAAQ00145

MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or of MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #OAAQ00145.

Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 ug/mL Phospho-MKK3 (Ser189)MKK6(S207) antibody MKK3S189MKK6S207-D3. Cat. #OAAQ00145.

Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV+TPA (green) using 0.5 ug/mL of Phospho-MKK3(Ser189)/MKK6(Ser207) antibody MKK3S189MKK6S207-D3 Cat. #OAAQ00145.

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 0.1ug/mL. Cat. # OAAQ00145.

Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 ug/mL Phospho-MKK3 (Ser189)MKK6(S207) antibody MKK3S189MKK6S207-D3. Cat. #OAAQ00145.

MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 ug/mL of isotype control Cat. #OAAQ00101 (orange) or of MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #OAAQ00145.

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 0.1ug/mL. Cat. # OAAQ00145.

Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV+TPA (green) using 0.5 ug/mL of Phospho-MKK3(Ser189)/MKK6(Ser207) antibody MKK3S189MKK6S207-D3 Cat. #OAAQ00145.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA.
• Clonality: Monoclonal
• Clone: MKK3S189MKK6S207-D3
• Isotype: Rabbit IgG1k
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC, WB
• Reconstitution and Storage: -20C
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.
• Purification: Protein A+G
• Target Post-Translational Modification: Phospho-Ser189/Ser207
• Application Info: 1ug/mL - 0.001ug/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reference: 1. Derijard, B. et al. (1995) Science 267, 682-685.
  2. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
  3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
  4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
  5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Target Info

• Gene Symbol: MAP2K3
• Gene Full Name: mitogen-activated protein kinase kinase 3
• Alias Symbols: MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2
• NCBI Gene Id: 5606
• Protein Name: dual specificity mitogen-activated protein kinase kinase 3
• Description of Target: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
• Uniprot ID: P46734
• Protein Accession #: NP_001303261.1
• Nucleotide Accession #: NM_001316332.1