Size:96 Wells
$279.00
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SKU
OKEH02965
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LHB ELISA Kit (Rat) (OKEH02965)

Catalog#: OKEH02965
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology LHB ELISA Kit (Rat) (OKEH02965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lhb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lhb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lhb captured in well.
Kit Range 0.156 - 10ng/mL
Kit Component
ComponentAmount
Anti-Lhb Microplate96 Wells (12 x 8 Well strips)
Lhb Lyophilized Standard2
100X Biotinylated Lhb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.087 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Rat Lutropin subunit beta
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol LHB
Official Gene Full Name luteinizing hormone beta polypeptide
Alias Symbols LH-B, LSH-B, LSH-beta, Luteinizing hormone subunit beta, Lutropin beta chain, Lutropin subunit beta
NCBI Gene Id 25329
Protein Name Lutropin subunit beta
Description of Target Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Swissprot Id P01230
Protein Accession # NP_001029147.1
Nucleotide Accession # NM_001033975.1
Protein Size (# AA) 141
Molecular Weight 15 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Lhb.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Lhb.
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