Search Antibody, Protein, and ELISA Kit Solutions

LHB ELISA Kit (Rat) (OKEH02965)

96 Wells
$389.00
In Stock
Request Bulk Order Quote

Gene Symbol:
LHB
Official Gene Full Name:
luteinizing hormone beta polypeptide
NCBI Gene Id:
25329
Alias Symbols:
LH-B, LSH-B, LSH-beta, Luteinizing hormone subunit beta, Lutropin beta chain, Lutropin subunit beta
Description of Target:
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Protein Name:
Lutropin subunit beta
Swissprot Id:
P01230
Protein Accession #:
NP_001029147.1
Nucleotide Accession #:
NM_001033975.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology LHB ELISA Kit (Rat) (OKEH02965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lhb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Lhb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lhb captured in well.
Kit Component:
ComponentAmount
Anti-Lhb Microplate96 Wells (12 x 8 Well strips)
Lhb Lyophilized Standard2
100X Biotinylated Lhb Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Lhb.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Lhb.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat Lutropin beta chain. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
15 kDa
Protein Size (# AA):
141

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...