IGFBP4 ELISA Kit (Cattle) (OKCD02621)

Catalog No: OKCD02621

Size:96WELLS

IGFBP4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	80-96%	79-101%	86-93%
  EDTA Plasma (n=5)	87-99%	94-101%	84-91%	83-96%
  Heparin Plasma (n=5)	78-89%	87-95%	88-102%	79-91%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and unlabeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) (Standards or samples) with the pre-coated antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample.
• ELISA Kit Range: 74.07-6000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-94	87
  EDTA Plasma (n=5)	78-93	89
  Heparin Plasma (n=5)	86-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
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• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 29.62 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: IGFBP4
• Gene Full Name: insulin like growth factor binding protein 4
• Alias Symbols: IBP-4;IGF-binding protein 4;IGFBP-4;insulin-like growth factor-binding protein 4.
• NCBI Gene Id: 282262
• Protein Name: Insulin-like growth factor-binding protein 4
• Description of Target: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
• Uniprot ID: Q05716
• Protein Accession #: NP_776982.1
• Nucleotide Accession #: NM_174557.4
• Molecular Weight: 258 kDa