Catalog No: OKEH00086
Size:96T
Price: $525.00
SKU
OKEH00086
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IGFBP4 ELISA Kit (Human) : 96 Wells (OKEH00086)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IGFBP4 ELISA Kit (Human) (OKEH00086) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP4 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
IGFBP4 Microplate96 Wells (12 x 8 Well strips)
IGFBP4 Lyophilized Standard2
100X Biotinylated IGFBP4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human Insulin-like growth factor-binding protein 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIGFBP4
Gene Full Nameinsulin like growth factor binding protein 4
Alias SymbolsBP-4;HT29-IGFBP;IBP4;IBP-4;IGF-binding protein 4;IGFBP-4;insulin-like growth factor-binding protein 4.
NCBI Gene Id3487
Protein NameP22692
Description of TargetInsulin-like growth factor-binding protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/P22692
Protein Accession #NP_001543
Nucleotide Accession #NM_001552
Protein Size (# AA)258
Molecular Weight28 kDa
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