Size:96 Wells
$389.00
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SKU
OKCD00785
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IGFBP4 ELISA Kit (Human) (OKCD00785)

Catalog#: OKCD00785
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
This product is Genway GWB-KBBQW2
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD 450 nm
Kit Duration 2.5 Hours
Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)94-103%80-103%98-105%93-102%
EDTA plasma(n=5)80-89%94-101%96-104%88-99%
heparin plasma(n=5)92-99%79-101%86-101%99-105%
Kit Principle Aviva Systems Biology IGFBP4 ELISA Kit (Human) (OKCD00785) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IGFBP4captured in well.
Kit Range 0.312-20 ng/mL
Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)91-9894
EDTA plasma(n=5)80-9184
heparin plasma(n=5)86-9390
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level IGFBP4 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level IGFBP4 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Component
ComponentAmount
Anti-IGFBP4 Microplate96 Wells (12 x 8 Well Strips)
IGFBP4 Lyophilized Standard2 x 20 ng
100X Biotinylated IGFBP4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity < 0.129 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich Immunoassay
Gene Symbol IGFBP4
Official Gene Full Name insulin like growth factor binding protein 4
Alias Symbols BP-4, HT29-IGFBP, IBP4, IBP-4, IGF-binding protein 4, IGFBP-4, Insulin-like growth factor-binding protein 4
NCBI Gene Id 3487
Protein Name insulin-like growth factor-binding protein 4
Description of Target IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Swissprot Id P22692
Protein Accession # NP_001543
Nucleotide Accession # NM_001552.2
Protein Size (# AA) 258
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IGFBP4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IGFBP4.
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