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Datasheets/Manuals | Printable datasheet for OASE00170 |
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Predicted Species Reactivity | Human, Mouse, Rat, Rabbit |
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Clonality | Monoclonal |
Clone | N114/10 (Formerly sold as S114-10) |
Isotype | IgG1 |
Host | Mouse |
Conjugation | Unconjugated |
Application | WB, IHC, ICC, IF, AM, FACS |
Additional Information | Background Info: Detects ~130kDa. No cross-reactivity against other HCNs |
:: | Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of HCN4 in 10ug of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | Store at -20C. Shipping with Blue Ice or 4C. |
Immunogen | Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4 |
Purification | Protein G Purified |
Concentration | 1 mg/ml |
Specificity | Detects ~130kDa. No cross-reactivity against other HCNs. |
Dilution | WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user. |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
Reference | 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232 |
Description | Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (1). |
Gene Symbol | HCN4 |
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Gene Full Name | hyperpolarization activated cyclic nucleotide-gated potassium channel 4 |
Alias Symbols | Hyperpolarization activated cyclic nucleotide gated potassium channel 4, HCN 4, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 |
NCBI Gene Id | 59266 |
Uniprot ID | Q9JKA7 |
Protein Accession # | NP_067690.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HCN4 Antibody (OASE00170)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Rabbit".
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How long will it take to receive "HCN4 Antibody (OASE00170)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "HCN4 Antibody (OASE00170)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HCN4 Antibody (OASE00170)"?
This target may also be called "Hyperpolarization activated cyclic nucleotide gated potassium channel 4, HCN 4, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4" in publications.
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What is the shipping cost for "HCN4 Antibody (OASE00170)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HCN4 Antibody (OASE00170)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HCN4 Antibody (OASE00170)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HCN4 Antibody (OASE00170)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HCN4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HCN4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HCN4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HCN4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HCN4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HCN4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.