GPC3 ELISA Kit (Mouse) (OKCA02389)

Catalog No: OKCA02389

Size:96 Wells

Mouse GPC3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	92	84-95
  1:2	94	84-98
  1:4	94	85-97
  1:8	90	84-94

• ELISA Kit Principle: Aviva Systems Biology Gpc3 ELISA Kit (Mouse) (OKCA02389) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Gpc3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Gpc3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Gpc3 captured in the well.
• ELISA Kit Range: 3.12 - 200 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	99	91-103
  EDTA Plasma (n=4)	95	90-99

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Gpc3 Microplate: 96 Wells (12 x 8 Well strips)
  • Gpc3 Lyophilized Standard: 2
  • 100X Biotinylated Gpc3 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates
• Sensitivity: 0.78 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GPC3
• Alias Symbols: OCI, OCI-5
• NCBI Gene Id: 14734
• Protein Name: Glypican-3
• Description of Target: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
• Uniprot ID: Q8CFZ4
• Protein Accession #: NP_057906
• Nucleotide Accession #: NM_016697