GPC3 Antibody - N-terminal region (OAAB16662)

Catalog No: OAAB16662 (Formerly GWB-K5S662)

Size:400ul

GPC3 Antibody(C35) (cat# OAAB16662) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

GPC3 Antibody(C35) (cat# OAAB16662) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

Glypican 3 (GPC3) Antibody (N-term) (Cat. # OAAB16662) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Glypican 3 (GPC3) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, ELISA
  Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
  Tissue Location: Highly expressed in lung, liver and kidney.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Glypican 3 (GPC3).
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 2 mg/ml
• Sample Type Confirmation:

  GPC3 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: GPC3
• Alias Symbols: SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2
• NCBI Gene Id: 2719
• Protein Name: Glypican-3
• Description of Target: GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
• Uniprot ID: P51654
• Protein Accession #: NP_004475.1
• Molecular Weight: 66 kDa