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Datasheets/Manuals | Printable datasheet for anti-ERBB2 (OASG04912) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat |
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Product Format | Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | IF |
:: | Cellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.; Isoform 2: Cytoplasm. Nucleus.; Isoform 3: Cytoplasm. Nucleus. Tissue Specificity: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived from human Neu around the phosphorylation site of T686. Location: 630-710aa. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Thr686 |
Specificity | Phospho-Neu (T686) Polyclonal Antibody detects endogenous levels of Neu protein only when phosphorylated at T686. |
Application Info | ELISA: 1:5000 IF: 1:200~1000 |
Gene Symbol | ERBB2 |
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Gene Full Name | erb-b2 receptor tyrosine kinase 2 |
Alias Symbols | NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu |
NCBI Gene Id | 2064; 13866; |
Description of Target | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. ; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. |
Uniprot ID | P04626, P70424, P06494 |
Molecular Weight | 150 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "ERBB2 Antibody (Phospho-Thr686) (OASG04912)" provided in?
This item is provided in "Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
This target may also be called "NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu" in publications.
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What is the shipping cost for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "150 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ERBB2 Antibody (Phospho-Thr686) (OASG04912)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ERBB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ERBB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ERBB2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ERBB2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ERBB2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ERBB2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.