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ERBB2 ELISA Kit (Rat) : 96 Wells (OKEH01330)

96 Wells
$469.00
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Predicted Species Reactivity:
Rat
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ERBB2 ELISA Kit (Rat) (OKEH01330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERBB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERBB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERBB2 captured in well.
Kit Range:
0.312 - 20ng/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Kit Reproducibility:
Mean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
Kit Component:
ComponentAmount
ERBB2 Microplate96 Wells (12 x 8 Well strips)
ERBB2 Lyophilized Standard2
100X Biotinylated ERBB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
ERBB2
Official Gene Full Name:
erb-b2 receptor tyrosine kinase 2
Alias Symbols:
Epidermal growth factor receptor-related protein, Neu, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2
NCBI Gene Id:
24337
Protein Name:
Receptor tyrosine-protein kinase erbB-2
Description of Target:
may play a role in cell proliferation and differentiation
Swissprot Id:
P06494
Protein Accession #:
NP_058699.2 
Nucleotide Accession #:
NM_017003.2
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.165 ng/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ERBB2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ERBB2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat Receptor tyrosine-protein kinase erbB-2
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
139 kDa
Protein Size (# AA):
1257

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