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Catalog No: OKEH01330
Size:96T
Price: $525.00
SKU
OKEH01330
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ERBB2 ELISA Kit (Rat) : 96 Wells (OKEH01330)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ERBB2 ELISA Kit (Rat) (OKEH01330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERBB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERBB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERBB2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
ELISA Kit Component
ComponentAmount
ERBB2 Microplate96 Wells (12 x 8 Well strips)
ERBB2 Lyophilized Standard2
100X Biotinylated ERBB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.165 ng/mL
SpecificityNatural and recombinant Rat Receptor tyrosine-protein kinase erbB-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolERBB2
Gene Full Nameerb-b2 receptor tyrosine kinase 2
Alias SymbolsEpidermal growth factor receptor-related protein;p185erbB2;p185neu;Proto-oncogene c-ErbB-2;Proto-oncogene Neu.
NCBI Gene Id24337
Protein NameP06494
Description of TargetReceptor tyrosine-protein kinase erbB-2
Uniprot IDhttps://www.uniprot.org/uniprot/P06494
Protein Accession #NP_058699.2 
Nucleotide Accession #NM_017003.2
Protein Size (# AA)1257
Molecular Weight139 kDa
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