Anti-C-erbB-2/HER-2 Antibody (OAAI00099)

Catalog No: OAAI00099

Size:100ug

Immuno-fluorescent staining: SK-BR-3 cells were fixed and immunoblotted by Mouse anti Her-2 (Cat#OAAI00099) incubation at a 1:250 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: S495
• Isotype: IgG1
• Host: Mouse
• Application: IHC-P, WB
• Additional Information: Background: Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
  Positive Control: SK-BR-3 cell lysate
  Cellular Location: Cell membrane.
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the C-terminus of Her2 protein. This sequence is identical in human, mouse, and ra
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Concentration: 100ug/200ul
• Specificity: ERBB2
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Xiao-Feng Le et al (2000) Anti-HER2 Antibody and Heregulin Suppress Growth of HER2-Overexpressing Human Breast Cancer Cells throμgh Different Mechanisms. Clinical Cancer Research Vol. 6, 260-270.

Target Info

• Gene Symbol: ERBB2
• Gene Full Name: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
• Alias Symbols: c-erbB2/neu protein
• NCBI Gene Id: 2064
• Description of Target: Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
  
• Uniprot ID: P04626
• Molecular Weight: 185 kDa