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Anti-C-erbB-2/HER-2 Antibody (OAAI00099)

100 ug
$305.00
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Predicted Species Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal
Isotype:
Mouse IgG1
Host:
Mouse
Application:
IHC, WB
Reconstitution and Storage:
The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity.
Avoid repeated freezing thawing cycles.
Replacement Item:
This antibody may replace item sc-08, HPA001383
Immunogen:
A synthetic peptide corresponding to the C-terminus of Her2 protein. This sequence is identical in Human, Mouse, and Rat.
Purification:
The Mouse IgG is purified by Isotyping-specific Affinity Purification.
Datasheets/Manuals:
Printable datasheet for Mouse Anti-C-erbB-2/HER-2 AntibodyOAAI00099
Specificity:
This antibody recognizes Her-2 protein. It cross-reacts with Human, Rat and Mouse. The other species was not tested.
Application Info:
Positive Control: SK-BR-3 Cell Lysate
Western Blot: 0.1-1 μg/ml
ELISA: 0.01-0.1 μg/ml
IP: 2-5 μg/ml
IHC: 2-5 μg/ml
Target Reference:
Xiao-Feng Le et al (2000) Anti-HER2 Antibody and Heregulin Suppress Growth of HER2-Overexpressing Human Breast Cancer Cells throμgh Different Mechanisms. Clinical Cancer Research Vol. 6, 260-270.
Gene Symbol:
ERBB2
Official Gene Full Name:
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
Alias Symbols:
NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu,
NCBI Gene Id:
2064
Description of Target:
Oncoprotein Her-2/Neu/ c-erbB-2 belongs to one of the four members of the ErbB receptor family. They are transmembrane receptor-like tyrosine kinases which can be autophosphorylated without ligand binding process when it is overexpressed in breast cancers. Her2 is an important biomarker in breast cancer diagnosis. The antibodies/ heregulin can suppress phosphorylation of HER2 on tyrosine Y1248. Endogenous anti-HER2 antibodies can effectively suppress HER2 kinase activity and downstream signaling to inhibit the transformed phenotype of HER2-expressing tumor cells. Thus, anti-Her2 antibodies have been used widely in the treatment of the breast cancer and other carcinomas.
Swissprot Id:
P04626
Molecular Weight:
~185 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ERBB2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ERBB2.

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