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Catalog No: OKEH02733
Price: $425.00
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ERBB2 ELISA Kit (Human) : 96 Wells (OKEH02733)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ERBB2 ELISA Kit (Human) (OKEH02733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERBB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ERBB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERBB2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
Anti-ERBB2 Microplate96 Wells (12 x 8 Well strips)
ERBB2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ERBB2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Receptor tyrosine-protein kinase erbB-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolERBB2
Gene Full Nameerb-b2 receptor tyrosine kinase 2
Alias SymbolsCD340;c-erb B2/neu protein;HER2;HER-2;HER-2/neu;herstatin;human epidermal growth factor receptor 2;metastatic lymph node gene 19 protein;MLN 19;NEU;neuro/glioblastoma derived oncogene homolog;neuroblastoma/glioblastoma derived oncogene homolog;NGL;p185erbB2;proto-oncogene c-ErbB-2;proto-oncogene Neu;receptor tyrosine-protein kinase erbB-2;TKR1;tyrosine kinase-type cell surface receptor HER2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2;v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;VSCN2.
NCBI Gene Id2064
Protein NameP04626
Description of TargetReceptor tyrosine-protein kinase erbB-2
Uniprot ID
Protein Accession #NP_001005862.1
Nucleotide Accession #NM_001005862.2
Protein Size (# AA)1255
Molecular Weight138 kDa
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