EGFR Antibody - N-terminal region (OAAB17145)

Catalog No: OAAB17145

Size:400ul

Western blot analysis of anti-ErbB1 Pab (Cat. #OAAB17145) in ZR-75-1 cell lysate. ErbB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand
  Tissue Location: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EGFR (ErbB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-57 amino acids from the N-terminal region of human EGFR (ErbB1).
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 1.7 mg/ml
• Application Info: IHC-P: 1:50~100
  WB: 1:1000

Target Info

• Gene Symbol: EGFR
• Alias Symbols: ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2
• NCBI Gene Id: 1956
• Protein Name: Epidermal growth factor receptor
• Description of Target: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
• Uniprot ID: P00533
• Protein Accession #: NP_005219.2
• Molecular Weight: 134 kDa