EGFR Antibody - N-terminal region (OAAB17145)

• Product Number: OAAB17145
• Name: EGFR Antibody - N-terminal region (OAAB17145)
• Molecular Weight: 134277
• Isotype: Ig
• NCBI Gene Id: 1956
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.7 mg/ml
• Gene Full Name: epidermal growth factor receptor
• Alias Symbols: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor tyrosine kinase domain;ERBB;ERBB1;erb-b2 receptor tyrosine kinase 1;ERRP;HER1;mENA;NISBD2;PIG61;proto-oncogene c-ErbB-1;receptor tyrosine-protein kinase erbB-1.
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Description of Target: Epidermal growth factor receptor, epidermal growth factor receptor isoform a precursor [Homo sapiens], epidermal growth factor receptor isoform b precursor [Homo sapiens], epidermal growth factor receptor isoform c precursor [Homo sapiens], epidermal growth factor receptor isoform d precursor [Homo sapiens]
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Note=In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand
  Tissue Location: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers
• Application Info: IHC-P: 1:50~100
  WB: 1:1000
• Immunogen: This EGFR (ErbB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-57 amino acids from the N-terminal region of human EGFR (ErbB1).
• Uniprot ID: https://www.uniprot.org/uniprot/P00533
• Protein Name: NP_005219.2, NP_958439.1, NP_958440.1, NP_958441.1, P00533
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_005219.2,NP_958439.1,NP_958440.1,NP_958441.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: EGFR
• Predicted Species Reactivity: Human
• Application: Immunohistochemistry-Paraffin, Western blot
• Image 1: ZR-75-1 cell
  
  Western blot analysis of anti-ErbB1 Pab (Cat. #OAAB17145) in ZR-75-1 cell lysate. ErbB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
• Image 2: human cancer tissue
  
  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.