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Datasheets/Manuals | Printable datasheet for EGFR Antibody (Phospho-Thr693) (OAAF00008) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr693. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | EGFR (phospho-Thr693) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 693. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000 |
Reference | Roland Reinehr, FASEB J, Feb 2003; 10: 1096. Natsumi Yamamoto, J. Cell Sci., Nov 2006; 119: 4623 - 4633. James G. Christensen Clin, Cancer Res., Dec 2001; 7: 4230. Hideki Katayama, AACR Meeting Abstracts, Mar 2004; 2004: 1074. Ali Tadlaoui |
Gene Symbol | EGFR |
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Gene Full Name | epidermal growth factor receptor |
Alias Symbols | avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor tyrosine kinase domain;ERBB;ERBB1;erb-b2 receptor tyrosine kinase 1;ERRP;HER1;mENA;NISBD2;PIG61;proto-oncogene c-ErbB-1;receptor tyrosine-protein kinase erbB-1. |
NCBI Gene Id | 1956 |
Protein Name | Epidermal growth factor receptor |
Description of Target | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance (By similarity). |
Uniprot ID | P00533 |
Protein Accession # | NP_005219.2 |
Molecular Weight | 134 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EGFR Antibody (Phospho-Thr693) (OAAF00008)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
This target may also be called "avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;cell growth inhibiting protein 40;cell proliferation-inducing protein 61;epidermal growth factor receptor;epidermal growth factor receptor tyrosine kinase domain;ERBB;ERBB1;erb-b2 receptor tyrosine kinase 1;ERRP;HER1;mENA;NISBD2;PIG61;proto-oncogene c-ErbB-1;receptor tyrosine-protein kinase erbB-1." in publications.
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What is the shipping cost for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "134 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EGFR Antibody (Phospho-Thr693) (OAAF00008)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EGFR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EGFR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EGFR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EGFR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EGFR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EGFR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.