Catalog No: OKCD08443
Size:96WELLS
Price: $850.00
SKU
OKCD08443
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for CYP1A1 ELISA Kit (Rat) (OKCD08443) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A1 (CYP1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A1 (CYP1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A1 (CYP1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 1A1 (CYP1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.121ng/mL |
Gene Symbol | Cyp1a1 |
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Gene Full Name | cytochrome P450, family 1, subfamily a, polypeptide 1 |
Alias Symbols | AHH;AHRR;aryl hydrocarbon hydroxylase;CP11;CYP1;Cyp45c;Cypc45c;CYPIA1;cytochrome P1-450;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c);cytochrome P450, 1a1;cytochrome P450, subfamily I (aromatic compound-inducible), member A1 (C6, form c) (C6, form c);cytochrome P450-C;cytochrome P450MT2;cytochrome P450-P1;dioxin-inducible;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P1-450;P450-C;P450DX;P450form6;P-450MC;P450MT2;xenobiotic monooxygenase. |
NCBI Gene Id | 24296 |
Protein Name | Cytochrome P450 1A1 |
Description of Target | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
Uniprot ID | P00185 |
Protein Accession # | NP_036672.2 |
Nucleotide Accession # | NM_012540.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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