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| Datasheets/Manuals | Printable datasheet for CYP1A1 Antibody (OABB02027) |
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| Tested Species Reactivity | Human, Mouse, Rat |
|---|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
| Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clonality | Polyclonal |
| Clone | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot |
| Additional Information | Notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
| :: | Background: CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation. |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1. |
| Purification | Affinity Purified |
| Concentration | 500 ug/ml |
| Specificity | No cross reactivity with other proteins. |
| Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human, Mouse Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
| Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Description | Rabbit IgG polyclonal antibody for Cytochrome P450 1A1(CYP1A1) detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM in Human;Mouse;Rat. |
| Gene Symbol | CYP1A1 |
|---|---|
| Gene Full Name | cytochrome P450 family 1 subfamily A member 1 |
| Alias Symbols | AHH;AHRR;aryl hydrocarbon hydroxylase;CP11;CYP1;CYPIA1;cytochrome P1-450, dioxin-inducible;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450, family 1, subfamily A, polypeptide 1;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1;cytochrome P450-C;cytochrome P450-P1;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;P1-450;P450-C;P450DX;xenobiotic monooxygenase. |
| NCBI Gene Id | 1543 |
| Protein Name | Cytochrome P450 1A1 |
| Description of Target | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. |
| Uniprot ID | P04798 |
| Molecular Weight | 58165 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CYP1A1 Antibody (OABB02027)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CYP1A1 Antibody (OABB02027)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CYP1A1 Antibody (OABB02027)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CYP1A1 Antibody (OABB02027)"?
This target may also be called "AHH;AHRR;aryl hydrocarbon hydroxylase;CP11;CYP1;CYPIA1;cytochrome P1-450, dioxin-inducible;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450, family 1, subfamily A, polypeptide 1;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1;cytochrome P450-C;cytochrome P450-P1;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;P1-450;P450-C;P450DX;xenobiotic monooxygenase." in publications.
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What is the shipping cost for "CYP1A1 Antibody (OABB02027)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CYP1A1 Antibody (OABB02027)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CYP1A1 Antibody (OABB02027)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "58165 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CYP1A1 Antibody (OABB02027)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CYP1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CYP1A1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CYP1A1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CYP1A1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CYP1A1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CYP1A1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
