Catalog No: OKEH02058
Size:96T
Price: $525.00
SKU
OKEH02058
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CYP1A1 ELISA Kit (Human) (OKEH02058) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP1A1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.1% (n = 20) Mean Inter-assay CV%: < 9.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.096 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Cytochrome P450 1A1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CYP1A1 |
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Gene Full Name | cytochrome P450 family 1 subfamily A member 1 |
Alias Symbols | AHH;AHRR;aryl hydrocarbon hydroxylase;CP11;CYP1;CYPIA1;cytochrome P1-450, dioxin-inducible;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450, family 1, subfamily A, polypeptide 1;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1;cytochrome P450-C;cytochrome P450-P1;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;P1-450;P450-C;P450DX;xenobiotic monooxygenase. |
NCBI Gene Id | 1543 |
Protein Name | P04798 |
Description of Target | Cytochrome P450 1A1 |
Uniprot ID | https://www.uniprot.org/uniprot/P04798 |
Protein Accession # | NP_000490.1 |
Nucleotide Accession # | NM_000499.4 |
Protein Size (# AA) | 512 |
Molecular Weight | 58 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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