Catalog No: OKEH02058
Size:96T
Price: $525.00
SKU
OKEH02058
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CYP1A1 ELISA Kit (Human) : 96 Wells (OKEH02058)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02058
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP1A1 ELISA Kit (Human) (OKEH02058) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP1A1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
CYP1A1 Microplate96 Wells (12 x 8 Well strips)
CYP1A1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PON2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.096 ng/mL
SpecificityNatural and recombinant Human Cytochrome P450 1A1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCYP1A1
Gene Full Namecytochrome P450 family 1 subfamily A member 1
Alias SymbolsAHH;AHRR;aryl hydrocarbon hydroxylase;CP11;CYP1;CYPIA1;cytochrome P1-450, dioxin-inducible;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450, family 1, subfamily A, polypeptide 1;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1;cytochrome P450-C;cytochrome P450-P1;flavoprotein-linked monooxygenase;hydroperoxy icosatetraenoate dehydratase;P1-450;P450-C;P450DX;xenobiotic monooxygenase.
NCBI Gene Id1543
Protein NameP04798
Description of TargetCytochrome P450 1A1
Uniprot IDhttps://www.uniprot.org/uniprot/P04798
Protein Accession #NP_000490.1
Nucleotide Accession #NM_000499.4
Protein Size (# AA)512
Molecular Weight58 kDa
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